Article

Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme

  1. Anton V. Filikov1,2,3,*,
  2. John R. Jones1,
  3. Nikolai F. Myasoedov2,
  4. E. Sally Ward3

Article first published online: 26 JUL 2006

DOI: 10.1002/jlcr.2580360210

Issue

Journal of Labelled Compounds and Radiopharmaceuticals

Journal of Labelled Compounds and Radiopharmaceuticals

Volume 36, Issue 2, pages 179–185, February 1995

Additional Information

How to Cite

Filikov, A. V., Jones, J. R., Myasoedov, N. F. and Ward, E. S. (1995), Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme. J Label Compd Radiopharm, 36: 179–185. doi: 10.1002/jlcr.2580360210

Author Information

  1. 1

    Chemistry Department, University of Surrey, Guildford GU2 5XH, Surrey, UK

  2. 2

    Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq., Moscow 123182, Russia

  3. 3

    Cancer Immunobiology Center and Department of Microbiology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-8576

*University of Texas

Publication History

  1. Issue published online: 26 JUL 2006
  2. Article first published online: 26 JUL 2006
  3. Manuscript Revised: 5 SEP 1994
  4. Manuscript Received: 2 SEP 1994

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Keywords:

  • isotope exchange;
  • proteins;
  • solid state hydrogenation. tritium;
  • deuterium

Abstract

Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi·mmol−1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.

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