Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme
Article first published online: 26 JUL 2006
Copyright © 1995 John Wiley & Sons, Ltd.
Journal of Labelled Compounds and Radiopharmaceuticals
Volume 36, Issue 2, pages 179–185, February 1995
How to Cite
Filikov, A. V., Jones, J. R., Myasoedov, N. F. and Ward, E. S. (1995), Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme. J Label Compd Radiopharm, 36: 179–185. doi: 10.1002/jlcr.2580360210
- Issue published online: 26 JUL 2006
- Article first published online: 26 JUL 2006
- Manuscript Revised: 5 SEP 1994
- Manuscript Received: 2 SEP 1994
- isotope exchange;
- solid state hydrogenation. tritium;
Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi·mmol−1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.