Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme

Authors

  • Anton V. Filikov,

    Corresponding author
    1. Chemistry Department, University of Surrey, Guildford GU2 5XH, Surrey, UK
    2. Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq., Moscow 123182, Russia
    3. Cancer Immunobiology Center and Department of Microbiology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-8576
    • University of Texas
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  • John R. Jones,

    1. Chemistry Department, University of Surrey, Guildford GU2 5XH, Surrey, UK
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  • Nikolai F. Myasoedov,

    1. Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq., Moscow 123182, Russia
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  • E. Sally Ward

    1. Cancer Immunobiology Center and Department of Microbiology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-8576
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Abstract

Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T2:H2 mixture of 1:1000, was 16 mCi·mmol−1. It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.

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