Is the adhesive material secreted by sea urchin tube feet species-specific?
Article first published online: 15 AUG 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Morphology
Volume 273, Issue 1, pages 40–48, January 2012
How to Cite
Santos, R. and Flammang, P. (2012), Is the adhesive material secreted by sea urchin tube feet species-specific?. J. Morphol., 273: 40–48. doi: 10.1002/jmor.11004
- Issue published online: 7 DEC 2011
- Article first published online: 15 AUG 2011
- Manuscript Accepted: 19 JUN 2011
- Manuscript Revised: 16 JUN 2011
- Manuscript Received: 25 MAY 2011
- Portuguese Foundation for Science and Technology
- Scientific Research of Belgium
- Fund for Scientific Research of Belgium. Grant Numbers: FRFC 2.4532.07, COST Action TD0906 (http://www.cost-bioadhesives.org/)
- temporary adhesion;
- tube feet;
- adhesive secretions;
- antibody cross-reactivity;
Sea urchin adoral tube feet are highly specialized organs that have evolved to provide efficient attachment to the substratum. They consist of a disk and a stem that together form a functional unit. Tube foot disk tenacity (adhesive force per unit area) and stem mechanical properties (e.g., stiffness) vary between species but are apparently not correlated with sea urchin taxa or habitats. Moreover, ultrastructural studies of sea urchin disk epidermis pointed out differences in the internal organization of the adhesive secretory granules among species. This prompted us to look for interspecific variability in the composition of echinoid adhesive secretions, which could explain the observed variability in adhesive granule ultrastructure and disk tenacity. Antisera raised against the footprint material of Sphaerechinus granularis (S. granularis) were first used to locate the origin of adhesive footprint constituents in tube feet by taking advantage of the polyclonal character of the generated antibodies. Immunohistochemical assays showed that the antibodies specifically labeled the adhesive secretory cells of the disk epidermis in the tube feet of S. granularis. The antibodies were then used on tube foot histological sections from seven other sea urchin species to shed some light on the variability of their adhesive substances by looking for antibody cross-reactivity. Surprisingly, no labeling was observed in any of the species tested. These results indicate that unlike the adhesive secretions of asteroids, those of echinoids do not share common epitopes on their constituents and thus would be “species-specific.” In sea urchins, variations in the composition of adhesive secretions could therefore explain interspecific differences in disk tenacity and in adhesive granule ultrastructure. J. Morphol., 2011. © 2011 Wiley Periodicals, Inc.