Morphology and SSU rRNA gene-based phylogeny of two Diophrys-like ciliates from northern china, with notes on morphogenesis of Pseudodiophrys nigricans (Protozoa, Ciliophora)

Authors

  • Yangbo Fan,

    1. The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
    2. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
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  • Alan Warren,

    1. Department of Zoology, Natural History Museum, London SW7 5BD, UK
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  • Saleh A. Al-Farraj,

    1. Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia
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  • Xumiao Chen,

    1. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
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  • Chen Shao

    Corresponding author
    1. The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
    • Chen Shao, The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
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Abstract

Two free-living marine euplotid ciliates, Pseudodiophrys nigricans and Diophrys japonica, collected from the coastal waters off Qingdao, northern China, were investigated using live observations and protargol impregnation methods. The cortical development of P. nigricans was observed during binary division. Although its general pattern of morphogenesis is similar to that of other Diophrys-like species, three unusual features are noteworthy: 1) the frontoventral transverse cirral anlagen develop in the secondary mode, similar to that of Euplotes; 2) the undulating membrane anlage migrates far from the cytostome and does not split into two membranes; and 3) the parental adoral zone of membranelles remains nearly intact throughout the entire morphogenetic process. Diophrys japonica is redescribed based on a Chinese population and can be recognized by having one left marginal cirrus, densely arranged cortical granules, and a fragment kinety with three dikinetids. Phylogenetic analyses based on the small subunit rRNA (SSU rRNA) gene sequence data indicate that D. japonica is placed within the Diophrys clade and is most closely related to the well-known D. apoligothrix. © J. Morphol., 2013. © 2012 Wiley Periodicals, Inc.

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