This article is published in Journal of Molecular Recognition as a special issue on Affinity 2009, edited by Gideon Fleminger, Tel-Aviv University, Tel-Aviv, Israel and George Ehrlich, Hoffmann-La Roche, Nutley, NJ, USA.
Amino acids–nucleotides biomolecular recognition: from biological occurrence to affinity chromatography†
Article first published online: 28 OCT 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Journal of Molecular Recognition
Special Issue: Affinity 2009 – The 18th biennial meeting of the International Society for Molecular Recognition
Volume 23, Issue 6, pages 505–518, November/December 2010
How to Cite
Sousa, F., Cruz, C. and Queiroz, J. A. (2010), Amino acids–nucleotides biomolecular recognition: from biological occurrence to affinity chromatography. J. Mol. Recognit., 23: 505–518. doi: 10.1002/jmr.1053
- Issue published online: 28 OCT 2010
- Article first published online: 28 OCT 2010
- Manuscript Accepted: 3 APR 2010
- Manuscript Revised: 5 FEB 2010
- Manuscript Received: 13 NOV 2009
- affinity chromatography;
- amino acid–base interactions;
- protein–DNA specific recognition;
- structural data
In this review, the protein–DNA interactions are discussed considering different perspectives, and the biological occurrence of this interaction is explained at atomic level. The evaluation of the amino acid–nucleotide recognition has been investigated analysing datasets for predicting the association preferences and the geometry that favours the interaction.
Based on this knowledge, an affinity chromatographic method was developed also exploiting this biological favoured contact. In fact, the implementation of this technique brings the possibility to apply the concept of molecular interactions to the development of new purification methodologies. In addition, the integration of the information recovered by all the different perspectives can bring new insights about some biological mechanisms, though not totally clarified. Copyright © 2010 John Wiley & Sons, Ltd.