This article is published as part of the AFM BioMed Conference on Life Sciences and Medicine, Paris 2011 of the Journal of Molecular Recognition, edited by Simon Scheuring, Pierre Parot and Jean-Luc Pellequer.
Atomic force microscopy characterization of silver nanoparticles interactions with marine diatom cells and extracellular polymeric substance†
Version of Record online: 19 APR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Molecular Recognition
Special Issue: AFM BioMed Conference on Life Sciences and Medicine, Paris 2011
Volume 25, Issue 5, pages 309–317, May 2012
How to Cite
Pletikapić, G., Žutić, V., Vinković Vrček, I. and Svetličić, V. (2012), Atomic force microscopy characterization of silver nanoparticles interactions with marine diatom cells and extracellular polymeric substance. J. Mol. Recognit., 25: 309–317. doi: 10.1002/jmr.2177
- Issue online: 19 APR 2012
- Version of Record online: 19 APR 2012
- Manuscript Accepted: 31 JAN 2012
- Manuscript Revised: 30 JAN 2012
- Manuscript Received: 23 DEC 2011
- Croatian Ministry of Science, Education and Sports. Grant Number: No. 0982934–2744
- marine diatom;
- Cylindrotheca closterium;
- Cylindrotheca fusiformis;
- silver nanoparticles;
- extracellular polymer substance;
- atomic force microscopy;
- diatom cell wall
This study highlights the capacity of atomic force microscopy (AFM) for investigating nanoparticle (NP) algal cell interaction with a subnanometer resolution. We designed a set of AFM experiments to characterize NP size, shape, and structure to visualize changes in the cell morphology induced by NPs and to characterize NP interaction with the extracellular polymeric substance (EPS). Samples for AFM imaging were prepared using the same protocol—drop deposition on mica and imaged in air. Here we address the interactions of Ag NPs with ubiquitous, lightly silicified marine diatoms Cylindrotheca fusiformis and Cylindrotheca closterium and their EPS. In natural seawater used throughout this study, the single Ag NPs adopted truncated tetrahedron morphology with particle heights of 10, 20, 30, and 40 nm. This size class Ag NPs penetrates the cell wall through the valve region built of silica NPs embedded in organic matrix. The Ag NPs cause a local damage inside the cell without disintegration of the cell wall. The EPS production has been shown to increase as a feedback response to Ag NP exposure and may contribute to detoxification mechanisms. Imaging EPS at high resolution revealed the incorporation of Ag NPs and their aggregates into the EPS–gel matrix, proving their detoxifying capacity. Copyright © 2012 John Wiley & Sons, Ltd.