The absence of tertiary interactions in a self-assembled DNA crystal structure
Article first published online: 20 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Molecular Recognition
Volume 25, Issue 4, pages 234–237, April 2012
How to Cite
Nguyen, N., Birktoft, J. J., Sha, R., Wang, T., Zheng, J., Constantinou, P. E., Ginell, S. L., Chen, Y., Mao, C. and Seeman, N. C. (2012), The absence of tertiary interactions in a self-assembled DNA crystal structure. J. Mol. Recognit., 25: 234–237. doi: 10.1002/jmr.2183
- Issue published online: 20 MAR 2012
- Article first published online: 20 MAR 2012
- Manuscript Accepted: 22 FEB 2012
- Manuscript Received: 20 JAN 2012
- self-assembled DNA crystals;
- DNA nanotechnology;
- crystal design;
- robust DNA motif
DNA is a highly effective molecule for controlling nanometer-scale structure. The convenience of using DNA lies in the programmability of Watson–Crick base-paired secondary interactions, useful both to design branched molecular motifs and to connect them through sticky-ended cohesion. Recently, the tensegrity triangle motif has been used to self-assemble three-dimensional crystals whose structures have been determined; sticky ends were reported to be the only intermolecular cohesive elements in those crystals. A recent communication in this journal suggested that tertiary interactions between phosphates and cytosine N(4) groups are responsible for intermolecular cohesion in these crystals, in addition to the secondary and covalent interactions programmed into the motif. To resolve this issue, we report experiments challenging this contention. Gel electrophoresis demonstrates that the tensegrity triangle exists in conditions where cytosine–PO4 tertiary interactions seem ineffective. Furthermore, we have crystallized a tensegrity triangle using a junction lacking the cytosine suggested for involvement in tertiary interactions. The unit cell is isomorphous with that of a tensegrity triangle crystal reported earlier. This structure has been solved by molecular replacement and refined. The data presented here leave no doubt that the tensegrity triangle crystal structures reported earlier depend only on base pairing and covalent interactions for their formation. Copyright © 2012 John Wiley & Sons, Ltd.