This article is published as part of the AFM BioMed Conference on Life Sciences and Medicine, Paris 2011 of the Journal of Molecular Recognition, edited by Simon Scheuring, Pierre Parot and Jean-Luc Pellequer.
Stiffness tomography exploration of living and fixed macrophages†
Article first published online: 19 APR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Molecular Recognition
Special Issue: AFM BioMed Conference on Life Sciences and Medicine, Paris 2011
Volume 25, Issue 5, pages 241–246, May 2012
How to Cite
Roduit, C., Longo, G., Benmessaoud, I., Volterra, A., Saha, B., Dietler, G. and Kasas, S. (2012), Stiffness tomography exploration of living and fixed macrophages. J. Mol. Recognit., 25: 241–246. doi: 10.1002/jmr.2184
- Issue published online: 19 APR 2012
- Article first published online: 19 APR 2012
- Manuscript Accepted: 22 FEB 2012
- Manuscript Revised: 21 FEB 2012
- Manuscript Received: 31 OCT 2011
- Indo-Swiss Joint Research Program (ISJRP). Grant Number: 122 941
- Swiss National Science Foundation. Grant Numbers: 200021-118147, CR32I3-130676
- stiffness tomography;
- mechanical properties;
Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We present here, for the first time, a description of the use of this technique to acquire detailed stiffness maps of fixed and living macrophages. Indeed, the mechanical properties of several macrophages were studied through stiffness tomography imaging, allowing some insight of the structures lying below the cell's surface. Through these investigations, we were able to evidence the presence and properties of stiff column-like features located underneath the cell membrane. To our knowledge, this is the first evidence of the presence, underneath the cell membrane, of such stiff features, which are in dimension and form compatible with phagosomes. Moreover, by exposing the cells to cytochalasin, we were able to study the induced modifications, obtaining an indication of the location and mechanical properties of the actin cytoskeleton. Copyright © 2012 John Wiley & Sons, Ltd.