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BIACORE J: a new platform for routine biomolecular interaction analysis

Authors

  • Rebecca L. Rich,

    1. Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA
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  • David G. Myszka

    Corresponding author
    1. Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA
    • Center for Biomolecular Interaction Analysis, School of Medicine, Room 4A417, University of Utah, 50 N Medical Drive, Salt Lake City, UT 84132, USA
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  • This article originally appeared in the 21st volume of Chemical Sensors (Chinese) and is reprinted here with permission.

Abstract

SPR biosensor technology continues to evolve. The recently released platform from Biacore AB (Uppsala, Sweden), BIACORE J, is designed for the routine analysis of biomolecular interactions. Using an antibody–protein A and a ligand–receptor system, we demonstrate the utility of BIACORE J in determining active concentration and binding affinities. The results from these studies illustrate the high sensitivity of the instrument and its ability to generate reproducible binding responses. The BIACORE J is easy to operate and useful in diverse applications, making SPR technology widely accessible as a research tool. Copyright © 2001 John Wiley & Sons, Ltd.

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