Journal of Molecular Recognition

Cover image for Vol. 26 Issue 1

January 2013

Volume 26, Issue 1

Pages 10–58

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Research Articles
    1. Issue Information (pages i–iii)

      Article first published online: 25 DEC 2012 | DOI: 10.1002/jmr.2225

  2. Research Articles

    1. Top of page
    2. Issue Information
    3. Research Articles
    1. Effect of CoCl2 on the content of different metals and a relative activity of DNA-hydrolyzing abzymes in the blood plasma of mice (pages 10–22)

      Galina A. Legostaeva, Nataliya P. Zaksas, Yordanka G. Gluhcheva, Sergey E. Sedykh, Maria E. Madzharova, Nina N. Atanassova, Valentina N. Buneva and Georgy A. Nevinsky

      Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2217

      Thumbnail image of graphical abstract

      Cobalt is a trace element, which is required for many biological processes but is toxic in high concentrations. The treatment of mice with CoCl2 did not change the relative content of Ca, Cu, and Zn but significantly increase the content of B (2.3-fold), Mg (1.5-fold), Al and Fe (2.1-fold), and Si (3.4-fold). IgGs of treated mice demonstrated lower activities than those for IgGs of non-treated mice either in the absence or in the presence of external metal ions..

    2. Mapping discontinuous protein-binding sites via structure-based peptide libraries: combining in silico and in vitro approaches (pages 23–31)

      Ines S. Jaeger, Ines Kretzschmar, Jana Körner, Armin A. Weiser, Carsten C. Mahrenholz, Ajish Potty, Katerina Kourentzi, Richard C. Willson, Rudolf Volkmer and Robert Preissner

      Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2237

      Thumbnail image of graphical abstract

      The detection of discontinuous binding sites of proteins still remains challenging, as they consist of several short segments that are not adjacent in the sequence but are in spatial proximity due to protein folding. To meet this challenge, we combined SUPERFICIAL with the SPOT synthesis. Discontinuous binding sites could be identified and peptides mimicking such sites could be generated. Such peptides represent a starting point to facilitate downstream applications such as drug or vaccine design or development of diagnostic tools.

    3. Glutathione S-transferase π complexes with and stimulates Na+,K+-ATPase (pages 32–37)

      Hideo Ochiai, Hiroshi Eguchi, Shunsuke Noguchi, Yutaro Hayashi, Hideaki Nishino, Masaru Kawamura and Chau H. Wu

      Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2238

      Thumbnail image of graphical abstract

      Glutathione S-transferase (GST) was found to complex with the Na+,K+-ATPase as shown by binding assay using quartz crystal microbalance. The complexation was obstructed by the addition of antiserum to the α-subunit of the Na+,K+-ATPase, suggesting specificity of complexation between GST and the Na+,K+-ATPase. GST stimulated the Na+,K+-ATPase activity up to 1.4-fold.

    4. Characterization of selenium-containing glutathione transferase zeta1–1 with high GPX activity prepared in eukaryotic cells (pages 38–45)

      Li Yin, Jian Song, Philip G. Board, Yang Yu, Xiao Han and Jingyan Wei

      Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2241

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      In this study, we have used directed mutagenesis and the inclusion of a selenocysteine (Sec) insertion sequence to engineer the expression in eukaryotic cells of human glutathione transferase zeta1-1 (hGSTZ1-1) with Sec in the active site (seleno-hGSTZ1-1). This modification converted hGSTZ1-1 into an active GPX and is the first time this has been achieved in eukaryotic cells.

    5. Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell (pages 46–50)

      Yongjun Li, Haiyan Qiao, Wei Yan, Jing Zhang, Chunyan Xing, Hongda Wang, Bailin Zhang and Jilin Tang

      Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2242

      Thumbnail image of graphical abstract

      Molecular recognition force spectroscopy (MR-FS) was applied to investigate the dynamic interaction between aptamer GBI-10 and tenascin-C (TN-C) on human glioblastoma cell surface. A series of kinetic parameters concerning interaction process were acquired. It was also found that this interaction depended on the presence of Mg2+.

    6. Halilectin 1 (H-1) and Halilectin 2 (H-2): two new lectins isolated from the marine sponge Haliclona caerulea (pages 51–58)

      Rômulo Farias Carneiro, Arthur Alves de Melo, Fernando Edson Pessoa do Nascimento, Clareane Avelino Simplicio, Kyria Santiago do Nascimento, Bruno Anderson Matias da Rocha, Silvana Saker-Sampaio, Raniere da Mata Moura, Sula Salani Mota, Benildo Sousa Cavada, Celso Shiniti Nagano and Alexandre Holanda Sampaio

      Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2243

      Thumbnail image of graphical abstract

      Two new lectins named H-1 and H-2 were isolated from the marine sponge Haliclona caerulea by using a combination of affinity chromatography and ion exchange chromatography. The hemagglutinating activity of H-1 could not be inhibited by any tested sugars, but H-2 was inhibited by glycoproteins O-linked. H-1 and H-2 exhibited dose-dependent toxicity against Artemia nauplii. Additionally, 76% of the primary structure of H-2 was determined by using mass spectrometry, H-2 showed similarity to translated genome contigs of marine sponge Amphimedon queenslandica.

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