Halilectin 1 (H-1) and Halilectin 2 (H-2): two new lectins isolated from the marine sponge Haliclona caerulea (pages 51–58)
Rômulo Farias Carneiro, Arthur Alves de Melo, Fernando Edson Pessoa do Nascimento, Clareane Avelino Simplicio, Kyria Santiago do Nascimento, Bruno Anderson Matias da Rocha, Silvana Saker-Sampaio, Raniere da Mata Moura, Sula Salani Mota, Benildo Sousa Cavada, Celso Shiniti Nagano and Alexandre Holanda Sampaio
Article first published online: 11 DEC 2012 | DOI: 10.1002/jmr.2243
Two new lectins named H-1 and H-2 were isolated from the marine sponge Haliclona caerulea by using a combination of affinity chromatography and ion exchange chromatography. The hemagglutinating activity of H-1 could not be inhibited by any tested sugars, but H-2 was inhibited by glycoproteins O-linked. H-1 and H-2 exhibited dose-dependent toxicity against Artemia nauplii. Additionally, 76% of the primary structure of H-2 was determined by using mass spectrometry, H-2 showed similarity to translated genome contigs of marine sponge Amphimedon queenslandica.