Journal of Molecular Recognition

Cover image for Vol. 27 Issue 1

January 2014

Volume 27, Issue 1

Pages i–iii, 1–63

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Review
    5. Research Articles
    1. Issue Information (pages i–iii)

      Article first published online: 17 DEC 2013 | DOI: 10.1002/jmr.2314

  2. Editorial

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Review
    5. Research Articles
  3. Review

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Review
    5. Research Articles
    1. You have full text access to this OnlineOpen article
      Systematic, spatial imaging of large multimolecular assemblies and the emerging principles of supramolecular order in biological systems (pages 3–18)

      Walter Schubert

      Article first published online: 17 DEC 2013 | DOI: 10.1002/jmr.2326

      Thumbnail image of graphical abstract

      Imaging cycler microscopy can directly visualize and quantify thousands of supermolecules in a single cell or a tissue section by molecular imaging beyond the spectral fluorescence resolution limit and at high spatial resolution (< 40 nm) without having to change hardware. A 20-year survey reveals that the detected toponome (the functional network code of biomolecules in cells and tissues) is a system of supermolecules with compositional and spatial periodicities constituting a major principle of supramolecular order in biological systems.

  4. Research Articles

    1. Top of page
    2. Issue Information
    3. Editorial
    4. Review
    5. Research Articles
    1. Design, synthesis and application of benzyl-sulfonate biomimetic affinity adsorbents for monoclonal antibody purification from transgenic corn (pages 19–31)

      Anastasios Maltezos, Dimitris Platis, Dimitrios Vlachakis, Sophia Kossida, Marigianna Marinou and Nikolaos E. Labrou

      Article first published online: 17 DEC 2013 | DOI: 10.1002/jmr.2327

      Thumbnail image of graphical abstract

      A limited affinity-ligand library was synthesized, immobilized, and evaluated for its ability to bind and purify recombinant mAb 2G12 expressed in transgenic corn.

      One ligand, bearing 4-aminobenzyl sulfonic acid linked on two chlorine atoms of the triazine ring (4ABS-Trz-4ABS), displayed high affinity (KD 0.2 μM) for mAb 2G12. Molecular modeling and molecular dynamics simulation studies were carried out to provide a detailed picture of the 4ABS-Trz-4ABS interaction with mAb 2G12.

      This affinity ligand was exploited for the development of a facile purification protocol for mAb 2G12.

    2. Why specific anti-integrase antibodies from HIV-infected patients can efficiently hydrolyze 21-mer oligopeptide corresponding to antigenic determinant of human myelin basic protein (pages 32–45)

      Elena S. Odintsova, Pavel S. Dmitrenok, Anna M. Timofeeva, Valentina N. Buneva and Georgy A. Nevinsky

      Article first published online: 17 DEC 2013 | DOI: 10.1002/jmr.2329

      Thumbnail image of graphical abstract

      All sites of cleavage of nonspecific 21-mer oligopeptide corresponding to antigenic determinant of human myelin basic protein (MBP) by anti-integrase abzymes from the sera of HIV-infected patients were studied. Detectable major, moderate, and minor products of this oligopeptide digestion by HIV-1 IgG (salt)mix, IgG(acid)mix, IgA(salt)mix, and IgM(acid)mix (eluted from MBP-Sepharose with salt or acidic buffer) are shown by long and short arrows, respectively, whereas minor ones are shown by diamonds.

    3. Label-free characterization of carbonic anhydrase—novel inhibitor interactions using surface plasmon resonance, isothermal titration calorimetry and fluorescence-based thermal shift assays (pages 46–56)

      Tiphaine Rogez-Florent, Laetitia Duhamel, Laurence Goossens, Perrine Six, Anne-Sophie Drucbert, Patrick Depreux, Pierre-Marie Danzé, David Landy, Jean-François Goossens and Catherine Foulon

      Article first published online: 17 DEC 2013 | DOI: 10.1002/jmr.2330

      Thumbnail image of graphical abstract

      In order to have a method for the screening and the thorough study of original inhibitors of human carbonic anhydrase II, performances of surface plasmon resonance (SPR) and fluorescence-based thermal shifts assays (TSA) were evaluated. Isothermal titration calorimetry was used to process TSA data and to validate SPR and TSA results. KD values obtained using these three label-free technologies match good. According to the low quantity of protein necessary in SPR for the screening of 140 compounds (10 µg) and to the possibility to obtain both affinity and kinetics data, this methodology appears as the most effective one.

    4. A selective molecularly imprinted polymer-carbon nanotube sensor for cotinine sensing (pages 57–63)

      Sadik Antwi-Boampong, Kristina S. Mani, Jean Carlan and Joseph J. BelBruno

      Article first published online: 17 DEC 2013 | DOI: 10.1002/jmr.2331

      Thumbnail image of graphical abstract

      The chemiresistor presented in this publication shows that molecular imprinting of poly-4-vinylphenol with cotinine yields polymeric scaffolds with cotinine-specific receptors that can discriminately bind cotinine.

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