Anastasios Maltezos, Dimitris Platis, Dimitrios Vlachakis, Sophia Kossida, Marigianna Marinou and Nikolaos E. Labrou
A limited affinity-ligand library was synthesized, immobilized, and evaluated for its ability to bind and purify recombinant mAb 2G12 expressed in transgenic corn.
One ligand, bearing 4-aminobenzyl sulfonic acid linked on two chlorine atoms of the triazine ring (4ABS-Trz-4ABS), displayed high affinity (KD 0.2 μM) for mAb 2G12. Molecular modeling and molecular dynamics simulation studies were carried out to provide a detailed picture of the 4ABS-Trz-4ABS interaction with mAb 2G12.
This affinity ligand was exploited for the development of a facile purification protocol for mAb 2G12.