MR microscopy of chick embryo vasculature

Authors

  • Bradley R. Smith PhD,

    1. Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Rm 141, Bryan Research Bldg. Research Dr, Durham, NC 27710
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  • Eric L. Effmann MD,

    1. Department of Radiology, Children's Hospital and Medical Center, Seattle
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  • G. Allan Johnson PhD

    Corresponding author
    1. Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Rm 141, Bryan Research Bldg. Research Dr, Durham, NC 27710
    • Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Rm 141, Bryan Research Bldg. Research Dr, Durham, NC 27710
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Abstract

Six-day-old chick embryos were examined with magnetic resonance microscopy after vascular perfusion fixation and perfusion with gadolinium-doped gelatin to highlight the developing vascular anatomy. Gadolinium gelatin, with its short T1, provided a source of signal contrast within the vessels. The entire embryo was embedded in gelatin to minimize susceptibility artifacts that are prevalent at the high field strength (7.0 T) used. A series of single-section spin-echo images were acquired with various TRs to determine the optimal imaging sequence for a three-dimensional (3D) acquisition. The combination of gadolinium gelatin in the vascular spaces, gelatin embedding of the specimen, and optimal acquisition parameters yielded a 3D stack of high-resolution images that was readily reconstructed and rendered to effectively demonstrate the developing thoracic vessels in the embryo.

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