1522-2586/asset/cover.gif?v=1&s=7744bbc5c5ee23bd9677f861606033d1d6742e48 "Journal of Magnetic Resonance Imaging")
The first two authors contributed equally to this work
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Original Research
Enhanced tumor MR imaging with gadolinium-loaded polychelating polymer-containing tumor-targeted liposomes
Article first published online: 24 JAN 2008
DOI: 10.1002/jmri.21202
Copyright © 2008 Wiley-Liss, Inc.
Additional Information
How to Cite
Erdogan, S., Medarova, Z. O., Roby, A., Moore, A. and Torchilin, V. P. (2008), Enhanced tumor MR imaging with gadolinium-loaded polychelating polymer-containing tumor-targeted liposomes. J. Magn. Reson. Imaging, 27: 574–580. doi: 10.1002/jmri.21202
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The first two authors contributed equally to this work
Publication History
- Issue published online: 28 FEB 2008
- Article first published online: 24 JAN 2008
- Manuscript Accepted: 5 SEP 2007
- Manuscript Received: 11 APR 2007
Funded by
- NIH. Grant Number: R01-EB002995
- Abstract
- Article
- References
- Cited By
Keywords:
- tumor imaging;
- MRI;
- liposome;
- polychelating amphihilic polymer;
- 2C5 antibody;
- T1 mapping
Abstract
Purpose
To significantly enhance tumor MR imaging by using a contrast agent combining three components—a long-circulating liposome, liposomal membrane-incorporated polychelating amphiphilic polymer heavily loaded with gadolinium, and cancer-specific monoclonal antibody 2C5 attached to the liposome surface.
Materials and Methods
Tumor-bearing animals were imaged prior and 4, 24, and 48 hours after i.v. injection of 2C5-modified and unmodified Gd-PAP-containing PEGylated liposomes. The faster and more specific accumulation of the novel contrast nanoparticles in tumors was also confirmed by 3D angiograms and by direct visualization of Gd-immunoliposomes in tumor sections by confocal microscopy.
Results
2C5-modified Gd-PAP-containing PEGylated liposomes allowed for fast and specific tumor imaging as early as 4 hours postinjection. T1 inversion recovery maps demonstrated a significant increase in tumor-associated R1 in animals injected with antibody-modified Gd-loaded liposomes 4 hours postinjection, followed by a gradual decrease consistent with clearance of the agent from the tumor region. In control animals injected with antibody-free liposomes the corresponding R1 values at all investigated timepoints were significantly smaller.
Conclusion
The results support the feasibility of using such multifunctional nanoparticular liposome-based agents simultaneously providing prolonged circulation, heavy Gd load, and specific cancer cell recognition as a superior contrast for MR tumor imaging. J. Magn. Reson. Imaging 2008. © 2008 Wiley-Liss, Inc.