The first two authors contributed equally to this work
Enhanced tumor MR imaging with gadolinium-loaded polychelating polymer-containing tumor-targeted liposomes
Article first published online: 24 JAN 2008
Copyright © 2008 Wiley-Liss, Inc.
Journal of Magnetic Resonance Imaging
Volume 27, Issue 3, pages 574–580, March 2008
How to Cite
Erdogan, S., Medarova, Z. O., Roby, A., Moore, A. and Torchilin, V. P. (2008), Enhanced tumor MR imaging with gadolinium-loaded polychelating polymer-containing tumor-targeted liposomes. J. Magn. Reson. Imaging, 27: 574–580. doi: 10.1002/jmri.21202
- Issue published online: 28 FEB 2008
- Article first published online: 24 JAN 2008
- Manuscript Accepted: 5 SEP 2007
- Manuscript Received: 11 APR 2007
- NIH. Grant Number: R01-EB002995
- tumor imaging;
- polychelating amphihilic polymer;
- 2C5 antibody;
- T1 mapping
To significantly enhance tumor MR imaging by using a contrast agent combining three components—a long-circulating liposome, liposomal membrane-incorporated polychelating amphiphilic polymer heavily loaded with gadolinium, and cancer-specific monoclonal antibody 2C5 attached to the liposome surface.
Materials and Methods
Tumor-bearing animals were imaged prior and 4, 24, and 48 hours after i.v. injection of 2C5-modified and unmodified Gd-PAP-containing PEGylated liposomes. The faster and more specific accumulation of the novel contrast nanoparticles in tumors was also confirmed by 3D angiograms and by direct visualization of Gd-immunoliposomes in tumor sections by confocal microscopy.
2C5-modified Gd-PAP-containing PEGylated liposomes allowed for fast and specific tumor imaging as early as 4 hours postinjection. T1 inversion recovery maps demonstrated a significant increase in tumor-associated R1 in animals injected with antibody-modified Gd-loaded liposomes 4 hours postinjection, followed by a gradual decrease consistent with clearance of the agent from the tumor region. In control animals injected with antibody-free liposomes the corresponding R1 values at all investigated timepoints were significantly smaller.
The results support the feasibility of using such multifunctional nanoparticular liposome-based agents simultaneously providing prolonged circulation, heavy Gd load, and specific cancer cell recognition as a superior contrast for MR tumor imaging. J. Magn. Reson. Imaging 2008. © 2008 Wiley-Liss, Inc.