Original Research

MRI-based large deformation high dimensional mapping of the hippocampus in rats: Development and validation of the technique

  1. R. Edward Hogan MD1,*,
  2. Viviane Bouilleret MD, PhD2,3,
  3. Ying Rui Liu2,
  4. Lei Wang PhD4,
  5. John P. Williams PhD5,
  6. Bianca Jupp PhD2,
  7. Damian Myers PhD2,
  8. Terence J. O'Brien MD2,6,7

Article first published online: 22 APR 2009

DOI: 10.1002/jmri.21766

Issue

Journal of Magnetic Resonance Imaging

Journal of Magnetic Resonance Imaging

Volume 29, Issue 5, pages 1027–1034, May 2009

Additional Information

How to Cite

Hogan, R. E., Bouilleret, V., Liu, Y. R., Wang, L., Williams, J. P., Jupp, B., Myers, D. and O'Brien, T. J. (2009), MRI-based large deformation high dimensional mapping of the hippocampus in rats: Development and validation of the technique. J. Magn. Reson. Imaging, 29: 1027–1034. doi: 10.1002/jmri.21766

Author Information

  1. 1

    The Department of Neurology, Washington University, St. Louis, Missouri

  2. 2

    Departments of Medicine, The Royal Melbourne Hospital, The University of Melbourne, Parkville, Victoria, Australia

  3. 3

    Department of Neurophysiology and Epilepsy, ApHp, CHU Bicetre, Paris, France

  4. 4

    Department of Psychiatry, Northwestern University, Chicago, Illinois

  5. 5

    The Howard Florey Institute, Parkville, Victoria, Australia

  6. 6

    Department of Surgery, The Royal Melbourne Hospital, The University of Melbourne, Parkville, Victoria, Australia

  7. 7

    The Royal Melbourne Hospital, The University of Melbourne, Parkville, Victoria, Australia

*Washington University in St. Louis, Department of Neurology, 660 S. Euclid Avenue, Campus Box 8111, St. Louis, MO 63110

Publication History

  1. Issue published online: 22 APR 2009
  2. Article first published online: 22 APR 2009
  3. Manuscript Accepted: 9 FEB 2009
  4. Manuscript Received: 29 SEP 2008

Funded by

  • Victorian Government Transport Accident Commission (TAC). Grant Number: DP0023

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Keywords:

  • MRI;
  • hippocampus;
  • rat

Abstract

Purpose

To report the detection of structural and functional biological changes in living animals using small animal in vivo MRI that complements traditional ex vivo histological techniques. We report the development and validation of the application of large deformation high dimensional mapping (HDM-LD) segmentation for the hippocampus in the rat.

Materials and Methods

High resolution volumetric T2 weighted MRI images were acquired at 4.7 Tesla from six male in-breed nonepileptic Wistar rats. Two HDM-LD segmentations of the hippocampus (automated 1 and automated 2) were compared with the manual segmentations of two investigators who independently segmented the hippocampi (manual 1 and manual 2).

Results

The mean overlap for the hippocampi between automated 1 and automated 2 for the right hippocampi was 94.4% (SD 1.0) and for the left hippocampi was 94.3% (SD 2.5), while the mean overlap between automated 1 and manual 1 for the right hippocampi was 91.4% (SD 1.3) and for the left hippocampi was 91.9% (SD 1.4). Mean values for absolute differences for comparisons of all the segmentations were the following: automated 1 versus automated 2, 3.2% (SD 1.0); manual 1 versus manual 2 6.82% (SD 5.22); automated 1 versus manual 1 13.0% (SD 1.8).

Conclusion

HDM-LD can be applied to obtain accurate and reproducible three-dimensional segmentations of the hippocampus from rat MR images. HDM-LD will be a useful tool for investigations of hippocampal structural changes in vivo in rat models of human disease. J. Magn. Reson. Imaging 2009;29:1027–1034. © 2009 Wiley-Liss, Inc.

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