In vivo vascular hallmarks of diffuse leukoaraiosis

Authors


Abstract

Purpose:

To characterize multiple patterns of vascular changes in leukoaraiosis using in vivo magnetic resonance imaging (MRI) techniques.

Materials and Methods:

We measured cerebral blood flow (CBF), cerebrovascular reactivity (CVR), and blood–brain-barrier (BBB) leakage in a group of 33 elderly subjects (age: 72.3 ± 6.8 years, 17 males, 16 females). Leukoaraiosis brain regions were identified in each subject using fluid-attenuated inversion-recovery (FLAIR) MRI. Vascular parameters in the leukoaraiosis regions were compared to those in the normal-appearing white matter (NAWM) regions. Vascular changes in leukoaraiosis were also compared to structural damage as assessed by diffusion tensor imaging.

Results:

CBF and CVR in leukoaraiosis regions were found to be 39.7 ± 5.2% (P < 0.001) and 52.5 ± 11.6% (P = 0.005), respectively, of those in NAWM. In subjects who did not have significant leukoaraiosis, CBF and CVR in regions with high risk for leukoaraiosis showed a slight reduction compared to the other white matter regions. Significant BBB leakage was also detected (P = 0.003) in leukoaraiosis and the extent of BBB leakage was positively correlated with mean diffusivity. In addition, CVR in NAWM was lower than that in white matter of subjects without significant leukoaraiosis.

Conclusion:

Leukoaraiosis was characterized by reduced CBF, CVR, and a leakage in the BBB. J. Magn. Reson. Imaging 2010;32:184–190. © 2010 Wiley-Liss, Inc.

LEUKOARAIOSIS is defined as diffuse white matter lesions that are often visible on computed tomography (CT) or magnetic resonance imaging (MRI) (1). In particular, these regions appear as hyperintense signals on fluid-attenuated inversion-recovery (FLAIR) MRI (2). Unlike white matter lesions associated with known causes such as stroke or brain tumor, the pathogenesis of leukoaraiosis is not well defined. Previous studies report that leukoaraiosis can be associated with various pathologic conditions including vascular dementia (3), Alzheimer's disease (4), hypertension (5–7), small vessel disease (8–10), atherosclerosis (11), and cerebral hemorrhage (12). Another common cause of leukoaraiosis is aging (13, 14). A large majority of elderly individuals have a certain degree of leukoaraiosis, which is most pronounced in the periventricular regions (15–19). There is also evidence that the amount of leukoaraiosis is predictive of cognitive function (20, 21). Therefore, it is important to characterize the physiologic mechanisms underlying age-related leukoaraiosis.

A number of studies have suggested a strong contribution of vascular dysfunction in leukoaraiosis associated with ischemic diseases. Pathologic studies of leukoaraiosis tissue have shown that these regions are characterized by arteriosclerosis and the thickening of the vascular wall as well as a narrowing of the vessel lumen (22, 23). Others have postulated that leukoaraiosis is caused by the toxicity of serum protein which leaks into the extravascular tissue when the blood–brain-barrier (BBB) is compromised (24–26). In the present study we aimed to assess whether vascular components also play an important role in age-related leukoaraiosis.

The brain vasculature in leukoaraiosis under in vivo conditions has previously been difficult to assess for two possible reasons. First, the perfusion values of white matter are intrinsically lower than that of gray matter (27), thus accurate measurement of white matter perfusion is not trivial. Second, diffuse leukoaraiosis lesions are small and the spatial resolution of the imaging modality needs to be sufficiently high in order to minimize partial volume effects from surrounding normal tissue. Recent advances in high-field MR systems as well as the development of novel MR sequences have allowed the mapping of various physiological parameters in the brain. In the present work we conducted a multiparametric assessment of vascular parameters to gain a better understanding of physiological hallmarks in leukoaraiosis in elderly subjects. Since we are interested in the pathogenesis of diffuse white matter lesions in the absence of clinically defined vascular diseases, we only selected participants who did not have overt cerebral infarction or lacunae.

In this cross-sectional study we first tested whether cerebral blood flow (CBF) in leukoaraiosis regions was reduced compared to normal-appearing white matter (NAWM). Considering that CBF reduction may not be specific to vascular damage, we also measured the vasodilatory capacity of the vessels using CO2 inhalation. As a control study, we assessed whether such deficits were also present in subjects who did not have significant leukoaraiosis. In another group of subjects, the leakage of the BBB was assessed using an MR contrast agent. We further assessed the correlation between the extent of BBB leakage and tissue structural integrity as measured by diffusion tensor imaging (DTI).

MATERIALS AND METHODS

Participants

This study enrolled 33 elderly subjects (age: 72.3 ± 6.8 years, 17 males, 16 females) who did not have brain tumor, dementia, history of stroke, or MRI evidence of cerebral infarction or lacunae. Other inclusion/exclusion criteria were: 1) no contraindication to MRI scanning (pacemaker, implanted metallic objects, neurostimulator); 2) general good health with no serious or unstable medical conditions including major neurological or psychiatric disorders; 3) age greater than 50. The Health Insurance Portability and Accountability Act (HIPAA) compliant protocol was approved by the Institutional Review Board and written informed consent was obtained from all subjects.

MRI Methods

The MRI scans were performed on a 3T MR system (Philips Medical Systems, Best, Netherlands) using a body coil for radiofrequency transmission and an 8-channel head coil with parallel imaging capability for signal reception.

Leukoaraiosis was assessed by hyperintense signals in FLAIR MRI. FLAIR images were acquired in the transverse plane with imaging parameters of field of view (FOV) = 230 × 230 mm2, acquisition resolution = 0.65 (anterior–posterior) × 0.87 (right–left) mm2 (k-space matrix size = 352 × 264), reconstructed resolution = 0.45 × 0.45 mm2, number of slices = 24, slice thickness = 5 mm (gap = 1 mm), TI = 2800 msec, TR/TE = 11000 msec/150 msec, echo train length = 27, SENSE factor = 2, and duration = 3.6 minutes.

Three vascular parameters were measured. Baseline CBF and cerebrovascular reactivity were determined in 17 subjects. BBB leakage was assessed in another group of 16 subjects.

Baseline CBF was measured using a pseudo-continuous arterial spin-labeling (PCASL) technique (28–30) with the following parameters: labeling duration = 1632 msec, TI = 1525 msec, labeling offset = −96 mm, TR/TE/flip angle = 3970msec/14msec/90°, SENSE factor = 2.5, number of controls/labels = 30 pairs, duration = 4 minutes. We used 17 transverse slices with 7 mm thickness, FOV of 240 × 240 mm2, and in-plane resolution of 3 × 3 mm2.

Cerebravascular reactivity (CVR) was assessed by the measurement of blood oxygenation level-dependent (BOLD) MRI signal before and during inhalation of 5% CO2 (mixed with 21% O2 and 74% N2). The measurement followed protocols established in Yezhuvath et al (31). Briefly, hypercapnia was administered via a Douglas bag (Harvard Apparatus, Holliston, MA) connected to a two-way valve. A research assistant was inside the magnet room throughout the experiment to switch the valve and to monitor the subject. Physiological parameters, including end-tidal (Et) CO2, breathing rate, heart rate, and arterial oxygenation (sO2), were monitored and recorded during the experiment (MEDRAD, Pittsburgh, PA; and Novametrix Medical Systems, Wallingford, CT). BOLD MR imaging used a single-shot gradient echo planar imaging (EPI) sequence with TR/TE/flip angle = 3000 msec/30 msec/90°, FOV = 220 × 220 mm2, in-plane resolution = 2.8 × 2.8 mm2, 25 transverse slices with 6 mm thickness, 141 volumes, and duration = 7 minutes.

BBB leakage was assessed by a contrast-enhanced vascular-space-occupancy (VASO) technique (27, 32), which is based on the difference between pre- and postcontrast VASO signal intensities. The scan protocol included a precontrast scan, an injection of a contrast agent by an MRI-compatible power injector (MEDRAD, Pittsburgh, PA), and a postcontrast scan. An FDA-approved contrast agent, Gd-DTPA (Magnevist), was used with a standard dosage (0.1 mmol/kg). The postcontrast VASO scan was initiated 2 minutes after the injection to allow the contrast agent to reach a steady state in the blood vessels. This technique has been previously used to study BBB leakage in different grades of glioma (33). The VASO imaging used a segmented spin echo EPI sequence with EPI factor = 7. Other imaging parameters were: FOV = 192 × 192 mm2, in-plane resolution = 1.5 × 1.5 mm2, k-space matrix size = 128 × 128, 32 coronal slices with 5 mm thickness, fold-over direction = right–left, inversion time = 1088 msec (34), TR/TE/flip angle = 6000 msec/3.4 msec/90, and duration = 2.5 minutes.

In addition, two structural MRI scans were performed. A DTI scan was performed using a single-shot spin echo EPI, 30 diffusion-encoding directions (35) with b-factor of 1000 s/mm2, TR/TE/flip angle = 5281msec/51msec/90°, 60 transverse slices, slice thickness = 2 mm (gap = 1 mm), FOV = 224 × 224 mm2, and in-plane resolution = 2 × 2 mm2. A T1-weighted high-resolution (1 × 1 × 1 mm3) image was acquired using a standard magnetization-prepared rapid acquisition of gradient echo (MPRAGE) sequence (36).

Data Analysis

Images from the vascular scans were spatially coregistered to the FLAIR images using Statistical Parametric Mapping (SPM, University College London, UK) software. Leukoaraiosis regions were identified from FLAIR images (Fig. 1e) using a semiautomatic method described previously (37). Briefly, the FLAIR images were skull-stripped and the voxels with signal intensity greater than 2 standard deviations above average were delineated as possible lesions. This was followed by manual editing to remove spurious voxels due to fat signal, motion effect, edge effect, or coil sensitivity inhomogeneity.

Figure 1.

Procedures for identification of NAWM ROI. a: T1 weighted image. b: White matter probability map obtained from the segmentation of T1-weighted image. Black and white voxels indicate 0 and 100% probability, respectively. c: Voxels with white matter probability greater than 90%. d: White matter lesions (blue voxels) overlaid on FLAIR image. e: FLAIR image. f: A preliminary NAWM ROI (red voxels) overlaid on FLAIR image. g–k: The preliminary NAWM ROI was shrunken iteratively for five times with each time removing one layer of voxels at the edge. This step is useful in minimizing partial voluming effect.

For comparison, regions of interest (ROIs) for NAWM were also obtained for each subject. Care was taken to avoid partial voluming with gray matter or lesion tissues. The identification of NAWM was conducted in three steps. In the first step, SPM was used to perform brain segmentation on the MPRAGE images (Fig. 1a), which yielded a white matter probabilistic map (Fig. 1b). The voxels with white matter probability greater than 90% were labeled (Fig. 1c). In the second step, the white matter lesion (Fig. 1d) voxels were removed from the white matter mask, resulting in a preliminary NAWM ROI (Fig. 1f). In the third step, potential partial-volumed voxels (for both gray-white partial voluming and normal-lesion partial voluming) were removed by iteratively shrinking the preliminary ROI by five times (Fig. 1g–k). Given that our voxel size was 0.45 mm, five iterations were chosen in order to shrink the ROI by 2.3 mm in every direction, which is comparable to the thickness of gray matter. We can therefore ensure that no gray matter was included in our final NAWM ROI (Fig. 1k).

Data processing for the three vascular imaging scans followed procedures previously established. CBF images were calculated from the difference between control and label images obtained from PCASL MRI (38, 39). CVR maps in units of %BOLD/mmHg EtCO2 were computed based on a linear regression between the EtCO2 trace and the BOLD signal time-course (31). For BBB leakage, the VASO signal difference between pre- and postcontrast images, denoted by ΔVASO, was obtained and written as percentage of equilibrium magnetization (27). As shown in previous studies (33), ΔVASO reflects a combined effect of cerebral blood volume (CBV) and BBB leakage. To separate these two components, the CBV effect was estimated from the group-averaged CBF data using the well-known CBV/CBF relationship (40), CBV = CBFα. Thus, the BBB leakage, KBBB, can be calculated by:

equation image(1)

where the subscripts L and N denote values in leukoaraiosis and NAWM, respectively. The value of α was chosen to be 0.38 based on the literature (40), but a range of other values were also tested. Note that the leakage in NAWM was assumed to be 0 in this calculation.

We used DTI studio (Johns Hopkins University, Baltimore, MD) for the analysis of DTI images. Images with diffusion weighting were registered to the image acquired without diffusion gradients to correct for distortions due to eddy currents. The diffusion tensor was then calculated for each voxel, from which mean diffusivity (MD) and fractional anisotropy (FA) were calculated.

The subjects in this study were divided into two groups based on whether the ratio between leukoaraiosis volume and intracranial volume is greater or smaller than 0.1%. Those with a value greater than 0.1% were referred to as “subjects with significant leukoaraiosis”; those with a value smaller than 0.1% were referred to as “subjects without significant leukoaraiosis.” For the former group, vascular parameters in leukoaraiosis regions were compared to those in the NAWM regions. For the latter group, vascular parameters in the high-risk regions were measured to assess the potential vascular deficit in the absence of FLAIR hyperintensity.

RESULTS

The volume of leukoaraiosis varied considerably among the elderly subjects studied (ranging from 0.012% to 1.194% of the intracranial volume). Ten subjects in the CBF/CVR study and 13 subjects in the BBB leakage study met our criterion for subjects with significant leukoaraiosis. Figure 2 shows the averaged parametric maps of CBF, CVR, ΔVASO, MD, and FA as well as the occurrence map of leukoaraiosis. Table 1 summarizes the comparison between parameters in leukoaraiosis and NAWM regions. A significant deficit was found for all three vascular parameters assessed.

Figure 2.

Averaged parametric maps of CBF, CVR, ΔVASO, MD, and FA. All images were spatially registered to a brain template and then averaged. For the ΔVASO images, part of the occipital and frontal pole was missing in a few subjects due to incomplete brain coverage. For these regions the ΔVASO map was based on the data available for the location. An occurrence map of leukoaraiosis is also shown. For each voxel the probabilistic value was calculated by dividing the number of subjects having leukoaraiosis at the location by the total number of subjects (n = 33). [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]

Table 1. Comparison of Physiological Parameters Between NAWM and Leukoaraiosis
ParameterNAWM (mean ± SEM)Leukoaraiosis (mean ± SEM)% change (mean ± SEM)P-valueN
  • *

    In the calculation of KBBB it was assumed that the leakage in NAWM is 0. Thus, this value in the table is 0 for all subjects.

CBF (ml/min/100 ml brain)33.5 ± 1.913.4 ± 2.0−60.3 ± 5.2< 0.00110
CVR (%BOLD/mmHg)0.066 ± 0.0040.035 ± 0.009−47.5 ± 11.60.00510
KBBB (%)0*0.42 ± 0.11N/A0.00313
MD (10−3 mm2/s)0.783 ± 0.0081.151 ± 0.03447.0 ± 3.9< 0.00113
FA0.400 ± 0.0050.240 ± 0.009−39.9 ± 2.2< 0.00113

In subjects without significant leukoaraiosis the CBF value in regions with a high risk for leukoaraiosis (the colored voxels in Fig. 2f) was found to be 23.6 ± 4.0 ml/min/100 ml (N = 7, mean ± SEM), which was significantly lower (P < 0.001) than that in the other white matter regions for the same subjects (32.1 ± 4.6). This finding suggests that these white matter regions have intrinsically lower CBF, which may explain why these regions have a higher probability to develop leukoaraiosis. It should be noted, however, that the extent of reduction (28.2 ± 2.2%) was milder when compared to the fully developed leukoaraiosis (Table 1). A similar finding was obtained from the CVR data. The high-risk regions had a lower CVR as compared to that in other white matter regions (P = 0.07).

These vascular changes were accompanied by structural damage, which was measured with MD and FA (Table 1). Furthermore, a significant correlation (r = 0.64, P = 0.019) was found between MD and KBBB (Fig. 3), suggesting a possible link between vascular damage and tissue structural integrity.

Figure 3.

Correlation between MD and KBBB across individuals (r = 0.64, P = 0.019; n = 13). KBBB was calculated using Eq. [1]. MD was obtained from DTI data.

NAWM in subjects with significant leukoaraiosis also showed signs of vascular damage. The CVR value in NAWM (0.066 ± 0.004 %BOLD/mmHg) was lower than that of equivalent regions in subjects without significant leukoaraiosis (0.082 ± 0.009 %BOLD/mmHg). Furthermore, among the subjects with significant leukoaraiosis, CVR in NAWM was correlated with leukoaraiosis volume (r = 0.63, P = 0.05) (Fig. 4). No correlations were found between leukoaraiosis volume and CBF, ΔVASON, MD, or FA in NAWM (P > 0.21).

Figure 4.

Correlation between CVR in NAWM and the leukoaraiosis volume (r = 0.63, P = 0.05; n = 10). The leukoaraiosis volume is presented in the percentage of the intracranial volume to account for brain size differences.

DISCUSSION

In this study we show in vivo evidence of a vascular dysfunction in leukoaraiosis. Such changes appear to include multiple domains of vascular properties. Both CBF, a parameter reflecting the blood supply to the tissue, and CVR, an index related to the vascular reserve, are reduced in leukoaraiosis regions, providing strong evidence that the condition is associated with vascular alterations. Apart from the ischemic component, the vascular damage also seems to involve the integrity of the BBB. A compromised BBB may allow the extravasation of serum protein and cause toxicity to tissue. Interestingly, both BBB integrity and vascular reserve are associated with endothelium function, suggesting a central role of vascular endothelium in the formation of leukoaraiosis, which is consistent with the recent hypothesis of O'Sullivan and colleagues (1, 41).

Our results confirmed findings from previous studies that report that CBF in leukoaraiosis is lower than that in NAWM (42–45). Furthermore, we showed that, even without leukoaraiosis, CBF in these high-risk regions is already lower than other white matter regions. Such a change was observed in the elderly subjects without significant leukoaraiosis, and was also confirmed in young healthy subjects (data not shown). This finding suggests that these white matter regions (often in deep brain tissue close to lateral ventricles; see Fig. 2f) have intrinsically low CBF, perhaps because they are located at the end of the vascular tree. Consequently, they are most susceptible to vascular deficits and the damage becomes more apparent with aging.

CBF in the gray matter is known to be coupled to energy demand and metabolism (46), which forms the basis of several brain mapping techniques such as functional MRI, positron emission tomography (PET), and optical imaging. Assuming that the coupling of blood flow and metabolic rate is also valid in the white matter, the observation of a reduced CBF in leukoaraiosis would not necessarily suggest vascular damage, since diminished metabolic demand in white matter (from axons and glial cells) may also cause a decrease in CBF (47). Therefore, in this study we further showed a CVR deficit in leukoaraiosis regions using hypercapnia as a vascular-specific challenge. The CVR deficit is particularly relevant in view of previous pathological findings of a thickening vascular wall (22, 23) and may be a more specific marker for leukoaraiosis (43, 48, 49). We also found that CVR in NAWM of the subjects with significant leukoaraiosis is already lower than that in white matter of subjects without significant leukoaraiosis and that there is a correlation between NAWM CVR and size of leukoaraiosis. These suggest that CVR appears to be an early event in the cascade of pathogenesis and may precede the manifestation of lesion.

Our observation of a reduced CBF and CVR in the regions that have a high risk for leukoaraiosis is in agreement with recent studies reporting that white matter areas with lower perfusion correspond to locations of higher occurrence frequency of leukoaraiosis (42, 50). A study in young healthy subjects (49) also reported that CVR is small or even negative in the high-risk regions. The findings that both CBF and CVR are compromised in the high-risk regions support the hypothesis that the pathogenesis of leukoaraiosis may be associated with ischemic conditions due to the episodic reduction of systemic arterial blood pressure (49, 51).

In this study, we also provided experimental evidence that vascular deficits may cause white matter lesions via mechanisms other than ischemia. Specifically, we showed that BBB leakage is also present in leukoaraiosis regions, which may result in a direct insult to the parenchyma tissue. We used the relationship between CBF and CBV, known as Grubb's equation (40), to estimate the BBB leakage from ΔVASO. The coefficient α was assumed to be 0.38, based on the existing literature (40). However, we tested other α values ranging from 0.2 to 0.6, and found that the estimated BBB leakage was still significant. BBB leakage in leukoaraiosis has been previously studied using MR contrast agent methods where it was demonstrated that subjects with more severe leukoaraiosis tend to show higher BBB leakage (52, 53). However, a direct comparison between leukoaraiosis and NAWM within the same subject has not been reported previously.

The structural changes in leukoaraiosis assessed by MD and FA are consistent with the previous findings (54). It has been suggested that tissue structural alteration assessed by DTI is linked to vascular dysfunction such as small vessel disease (55). Our data support this notion by showing that the BBB leakage is correlated with an increased MD.

The results of the present study should be interpreted in view of a few limitations. First, due to the complex procedures (eg, CO2 inhalation, IV preparation, contrast agent administration) and long duration of the scans, the MRI measures mentioned above were not acquired from the same subjects. Instead, CBF and CVR were studied in one group of subjects (n = 17), and BBB leakage and diffusion were studied in another group (n = 16). We note, however, that there is no age difference between the groups (P = 0.25). Second, the CBF measurement used ASL MRI and this technique is known to be sensitive to arterial transit time of the blood (56). Thus, it is possible that part of the CBF difference between leukoaraiosis and NAWM is due to the differences in blood transit time. Additionally, the sample size in the present study is moderate and the P-values reported were not corrected for multiple comparisons. A further study with a larger number of subjects would be useful to confirm these findings.

In conclusion, leukoaraiosis regions are characterized by various forms of vascular damages including reduced blood supply, vascular reserve, and significant leakage in BBB. In addition, there are early signs of vascular damage even in NAWM and CVR appears to be a sensitive marker to detect such changes.

Acknowledgements

The authors are grateful to Dr. Matthias van Osch for providing the ASL pulse sequence and Sina Aslan for assistance with the data processing. We also thank Dr. Nyaz Didehbani for proofreading.

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