Reproducibility of black blood dynamic contrast-enhanced magnetic resonance imaging in aortic plaques of atherosclerotic rabbits
Article first published online: 23 JUN 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Magnetic Resonance Imaging
Volume 32, Issue 1, pages 191–198, July 2010
How to Cite
Calcagno, C., Vucic, E., Mani, V., Goldschlager, G. and Fayad, Z. A. (2010), Reproducibility of black blood dynamic contrast-enhanced magnetic resonance imaging in aortic plaques of atherosclerotic rabbits. J. Magn. Reson. Imaging, 32: 191–198. doi: 10.1002/jmri.22225
- Issue published online: 23 JUN 2010
- Article first published online: 23 JUN 2010
- Manuscript Accepted: 6 APR 2010
- Manuscript Received: 16 JUN 2009
- National Institutes of Health. Grant Numbers: NHLBI HL71021, NHLBI RO1 HL 78667, NIBIB RO1 EB 009638 (to Z.A.F.)
- Founders Affiliate of the American Heart Association (postdoctoral fellowship to V.M.)
To investigate the short-term reproducibility of black-blood dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) in atherosclerotic rabbits to evaluate the potential of this technique to be a reliable readout of plaque progression and/or regression upon therapeutic intervention.
Materials and Methods:
Atherosclerotic rabbits were imaged at baseline and 24 hours later with DCE-MRI on a 1.5T MRI system. DCE-MRI images were analyzed by calculating the area under the signal intensity versus time curve (AUC). Intraclass correlation coefficients (ICCs) were used to evaluate interscan, intraobserver, and interobserver reproducibility. In addition, the test–retest coefficient of variation (CoV) was evaluated.
Statistical analyses showed excellent interscan, intraobserver, and interobserver agreement. All ICCs were greater than 0.75, P < 0.01 indicating excellent agreement between measurements.
Experimental results show good interscan and excellent intra- and interobserver reproducibility, suggesting that DCE-MRI could be used in preclinical settings as a read-out for novel therapeutic interventions for atherosclerosis. This preliminary work encourages investigating the reproducibility of DCE-MRI also in clinical settings, where it could be used for monitoring high-risk patients and in longitudinal clinical drug trials. J. Magn. Reson. Imaging 2010;32:191–198. © 2010 Wiley-Liss, Inc.