Interstitial MR lymphography in mice with gadopentetate dimeglumine and gadoxetate disodium

Authors

  • Fugeng Sheng MD,

    1. Department of Radiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    2. Department of Radiology, Affiliated Hospital of The Academy of Military Medical Sciences, Beijing, China
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  • Yusuke Inoue MD, PhD,

    Corresponding author
    1. Department of Radiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    2. Department of Diagnostic Radiology, Kitasato University School of Medicine, Sagamihara, Japan
    • Department of Diagnostic Radiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 252-0374, Japan
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  • Shigeru Kiryu MD, PhD,

    1. Department of Radiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
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  • Makoto Watanabe MD, PhD,

    1. Department of Radiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
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  • Kuni Ohtomo MD, PhD

    1. Department of Radiology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
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Abstract

Purpose

To investigate the utility of interstitial MR lymphography with gadopentetate dimeglumine (Gd-DTPA) or gadoxetate disodium (Gd-EOB-DTPA) in mice.

Materials and Methods

We performed MR lymphography after the subcutaneous injection of Gd-DTPA or Gd-EOB-DTPA (0.1, 0.5, or 2.0 μmol per mouse) into the right footpad in six healthy mice, and the time courses of contrast enhancement were assessed. Additionally, the lymphatic pathways from two distinct sites were assessed in tandem by interstitial MR lymphography studies.

Results

Subcutaneous injection of Gd-DTPA or Gd-EOB-DTPA caused lymph node enhancement immediately after injection, followed by a rapid decline. Dose dependency was shown for the lymph node enhancement, and a high-dose injection caused prominent visualization of the veins. Lymph node enhancement did not differ significantly between Gd-DTPA and Gd-EOB-DTPA or between Gd-EOB-DTPA premixed and not premixed with bovine serum albumin. The tandem assessment of two lymphatic pathways was feasible, and image fusion aided detailed comparison.

Conclusion

Interstitial MR lymphography with Gd-DTPA or Gd-EOB-DTPA allowed clear visualization of the lymphatic pathway in healthy mice, and no significant difference was found between the two agents. Their rapid kinetics limits the imaging timing window, however, facilitates repeated assessment in a single imaging session. J. Magn. Reson. Imaging 2011;33:490–497. © 2011 Wiley-Liss, Inc.

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