Characterization of the cardiac response to a low and high dose of dobutamine in the mouse model of dilated cardiomyopathy by MRI in vivo
Article first published online: 2 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Magnetic Resonance Imaging
Volume 37, Issue 3, pages 669–677, March 2013
How to Cite
Tyrankiewicz, U., Skorka, T., Jablonska, M., Petkow-Dimitrow, P. and Chlopicki, S. (2013), Characterization of the cardiac response to a low and high dose of dobutamine in the mouse model of dilated cardiomyopathy by MRI in vivo. J. Magn. Reson. Imaging, 37: 669–677. doi: 10.1002/jmri.23854
- Issue published online: 15 FEB 2013
- Article first published online: 2 NOV 2012
- Manuscript Accepted: 4 SEP 2012
- Manuscript Received: 18 NOV 2011
- Polish Ministry of Science and Higher Education. Grant Number: NN518419733 (2007-2010)
- European Union from the resources of the European Regional Development Fund under the Innovative Economy Programme (grant coordinated by JCET-UJ)
- Unknown funding agency. Grant Number: POIG.01.01.02-00-069/09
- cardiac ischemia;
- heart failure;
To assess the cardiac response to low (0.15–0.5 mg/kg i.p.) and high (1.5–20 mg/kg i.p.) doses of dobutamine in Tgαq*44 mice with dilated cardiomyopathy at the stage of advanced heart failure.
Materials and Methods:
Inotropic, lusitropic, and chronotropic response to β1-adrenergic stimulation was assessed by the cine magnetic resonance imaging (MRI) protocol based on the electrocardiogram (ECG)-triggered bright-blood images of one midventricular short-axis slice.
In wildtype mice increasing doses of dobutamine resulted in subsequent increase in the left ventricular function and heart rate acceleration, but significant inotropic, lusitropic, and chronotropic cardiac response was observed only after high doses of dobutamine, what is typical. In the Tgαq*44 mice low doses of dobutamine significantly increased inotropic and lusitropic cardiac performance without chronotropic changes. An increased heart rate was observed only after high doses of dobutamine, but then inotropic and lusitropic cardiac functional reserve was lost.
We described MRI stress test protocol based on a low and high dose of dobutamine induced response that proves useful in revealing alternation in cardiac function in mice with heart failure. J. Magn. Reson. Imaging 2013;37:669–677. © 2012 Wiley Periodicals, Inc.