This article is a US Government work and is in the public domain in the USA.
Fully automated liquid extraction-based surface sampling and ionization using a chip-based robotic nanoelectrospray platform†
Article first published online: 17 DEC 2009
This article is a US Government work and is in the public domain in the USA. Published in 2009 by John Wiley & Sons, Ltd.
Journal of Mass Spectrometry
Volume 45, Issue 3, pages 252–260, March 2010
How to Cite
Kertesz, V. and Van Berkel, G. J. (2010), Fully automated liquid extraction-based surface sampling and ionization using a chip-based robotic nanoelectrospray platform. J. Mass Spectrom., 45: 252–260. doi: 10.1002/jms.1709
- Issue published online: 5 MAR 2010
- Article first published online: 17 DEC 2009
- Manuscript Accepted: 16 NOV 2009
- Manuscript Received: 11 SEP 2009
- Battelle Memorial Institute Technology Maturation Fund
- surface sampling;
- mass spectrometry;
- dried blood spots;
- thin tissue sections;
- MALDI spots
A fully automated liquid extraction-based surface sampling device utilizing an Advion NanoMate chip-based infusion nanoelectrospray ionization system is reported. Analyses were enabled for discrete spot sampling by using the Advanced User Interface of the current commercial control software. This software interface provided the parameter control necessary for the NanoMate robotic pipettor to both form and withdraw a liquid microjunction for sampling from a surface. The system was tested with three types of analytically important sample surface types, viz., spotted sample arrays on a MALDI plate, dried blood spots on paper, and whole-body thin tissue sections from drug dosed mice. The qualitative and quantitative data were consistent with previous studies employing other liquid extraction-based surface sampling techniques. Published in 2009 by John Wiley & Sons, Ltd.