Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation
Article first published online: 20 JUL 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Journal of Mass Spectrometry
Volume 45, Issue 10, pages 1098–1103, October 2010
How to Cite
Parr, C. and Brodbelt, J. S. (2010), Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation. J. Mass Spectrom., 45: 1098–1103. doi: 10.1002/jms.1780
- Issue published online: 20 JUL 2010
- Article first published online: 20 JUL 2010
- Manuscript Accepted: 7 JUN 2010
- Manuscript Received: 16 NOV 2009
- Robert A. Welch Foundation. Grant Number: F-1155
- National Institutes of Health. Grant Number: RO1 GM65956
- infrared multiphoton dissociation;
- collision-induced dissociation;
Infrared multiphoton dissociation (IRMPD) of thymine-rich oligodeoxynucleotides in a linear ion-trap mass spectrometer affords far more extensive fragmentation than conventional collision-induced dissociation (CID). For oligodeoxynucleotides containing one non-thymine base, CID results primarily in cleavage on the 3′ side of the non-thymine nucleobase, whereas IRMPD results in cleavages between all the nucleobases and thus provides complete sequence coverage. Furthermore, for oligodeoxynucleotides containing a single non-thymine base, it is shown that the full series of diagnostic sequence ions observed in the IRMPD mass spectra arise from secondary dissociation of the two primary products formed from the initial cleavage site located next to the non-thymine base. Copyright © 2010 John Wiley & Sons, Ltd.