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Protein PTMs: post-translational modifications or pesky trouble makers?

Authors

  • Ralph A. Bradshaw,

    1. NCRR Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, UCSF, San Francisco, CA 94158, USA
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  • Katalin F. Medzihradszky,

    1. NCRR Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, UCSF, San Francisco, CA 94158, USA
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  • Robert J. Chalkley

    Corresponding author
    1. NCRR Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, UCSF, San Francisco, CA 94158, USA
    • Department of Pharmaceutical Chemistry, University of California San Francisco, 600 16th Street, Genentech Hall, N-472, San Francisco, CA 94158-2517, USA.
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Abstract

Analysis of protein post-translational modifications using mass spectrometry is an intensive area of proteomic research. This perspective discusses the current state of the field with respect to what can be achieved, the challenges encountered, most notably with modification site assignment, the reliability of the published results, consequences of unreliable data and what is needed to be done to improve the situation. Copyright © 2010 John Wiley & Sons, Ltd.

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