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Selected reaction monitoring applied to proteomics

  1. Sebastien Gallien,
  2. Elodie Duriez,
  3. Bruno Domon*

Article first published online: 10 MAR 2011

DOI: 10.1002/jms.1895

Issue

Journal of Mass Spectrometry

Journal of Mass Spectrometry

Volume 46, Issue 3, pages 298–312, March 2011

Additional Information

How to Cite

Gallien, S., Duriez, E. and Domon, B. (2011), Selected reaction monitoring applied to proteomics. J. Mass Spectrom., 46: 298–312. doi: 10.1002/jms.1895

Author Information

  1. Luxembourg Clinical Proteomics center (LCP), Centre de Recherche Public de la Santé, 1 B rue Thomas Edison, L-1445 Strassen, Luxembourg

*Luxembourg Clinical Proteomics center (LCP), Centre de Recherche Public de la Santé, 1 B rue Thomas Edison, L-1445 Strassen, Luxembourg.

Publication History

  1. Issue published online: 10 MAR 2011
  2. Article first published online: 10 MAR 2011
  3. Manuscript Accepted: 12 JAN 2011
  4. Manuscript Received: 3 NOV 2010

Funded by

  • PEARL

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Keywords:

  • selected reaction monitoring;
  • proteomics;
  • quantification;
  • triple quadrupole mass spectrometer;
  • multiplexing

Abstract

Selected reaction monitoring (SRM) performed on triple quadrupole mass spectrometers has been the reference quantitative technique to analyze small molecules for several decades. It is now emerging in proteomics as the ideal tool to complement shotgun qualitative studies; targeted SRM quantitative analysis offers high selectivity, sensitivity and a wide dynamic range. However, SRM applied to proteomics presents singularities that distinguish it from small molecules analysis. This review is an overview of SRM technology and describes the specificities and the technical aspects of proteomics experiments. Ongoing developments aiming at increasing multiplexing capabilities of SRM are discussed; they dramatically improve its throughput and extend its field of application to directed or supervised discovery experiments. Copyright © 2011 John Wiley & Sons, Ltd.

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