Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
Article first published online: 28 SEP 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Mass Spectrometry
Volume 46, Issue 10, pages 967–975, October 2011
How to Cite
Mandal, M. K., Chen, L. C., Yu, Z., Nonami, H., Erra-Balsells, R. and Hiraoka, K. (2011), Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS). J. Mass Spectrom., 46: 967–975. doi: 10.1002/jms.1977
- Issue published online: 28 SEP 2011
- Article first published online: 28 SEP 2011
- Manuscript Accepted: 2 AUG 2011
- Manuscript Revised: 8 JUL 2011
- Manuscript Received: 11 MAY 2011
- probe electrospray ionization;
- multiply charged protein ions;
Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents. Copyright © 2011 John Wiley & Sons, Ltd.