Research Article

Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)

  1. Mridul Kanti Mandal1,
  2. Lee Chuin Chen2,
  3. Zhan Yu1,3,
  4. Hiroshi Nonami4,
  5. Rosa Erra-Balsells5,
  6. Kenzo Hiraoka1,*

Article first published online: 28 SEP 2011

DOI: 10.1002/jms.1977

Issue

Journal of Mass Spectrometry

Journal of Mass Spectrometry

Volume 46, Issue 10, pages 967–975, October 2011

Additional Information

How to Cite

Mandal, M. K., Chen, L. C., Yu, Z., Nonami, H., Erra-Balsells, R. and Hiraoka, K. (2011), Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS). J. Mass Spectrom., 46: 967–975. doi: 10.1002/jms.1977

Author Information

  1. 1

    Clean Energy Research Center, University of Yamanashi, Kofu, Japan

  2. 2

    Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan

  3. 3

    College of Chemistry and Biology, Shenyang Normal University, Shenyang, China

  4. 4

    Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan

  5. 5

    CIHIDECAR–CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

*Kenzo Hiraoka, Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400–8511, Japan. E-mail: hiraoka@yamanashi.ac.jp

Publication History

  1. Issue published online: 28 SEP 2011
  2. Article first published online: 28 SEP 2011
  3. Manuscript Accepted: 2 AUG 2011
  4. Manuscript Revised: 8 JUL 2011
  5. Manuscript Received: 11 MAY 2011

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Keywords:

  • electrospray;
  • nano-electrospray;
  • probe electrospray ionization;
  • multiply charged protein ions;
  • detergents

Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents. Copyright © 2011 John Wiley & Sons, Ltd.

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