This article is part of the Journal of Mass Spectrometry special issue entitled “2nd MS Food Day” edited by Gianluca Giorgi.
A liquid chromatography-mass spectrometry approach to study “glucosinoloma” in broccoli sprouts†
Article first published online: 11 SEP 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Mass Spectrometry
Special Issue: 2nd MS Food Day
Volume 47, Issue 9, pages 1198–1206, September 2012
How to Cite
Maldini, M., Baima, S., Morelli, G., Scaccini, C. and Natella, F. (2012), A liquid chromatography-mass spectrometry approach to study “glucosinoloma” in broccoli sprouts. J. Mass Spectrom., 47: 1198–1206. doi: 10.1002/jms.3028
- Issue published online: 6 SEP 2012
- Article first published online: 11 SEP 2012
- Manuscript Accepted: 15 MAY 2012
- Manuscript Revised: 9 MAY 2012
- Manuscript Received: 25 JAN 2012
- broccoli sprouts;
- growth condition;
Glucosinolates are an important class of secondary plant metabolites, possessing health-promoting properties. Young broccoli plants are a very good source of glucosinolates with concentrations several times greater than in mature plants. The aim of our study was to develop a liquid chromatography–mass spectrometry and liquid chromatography/tandem mass spectrometry qualitative and quantitative method for the measure of glucosinolates in broccoli sprouts. The described method provides high sensitivity and specificity, allowing a rapid and simultaneous determination of 14 glucosinolates. The proposed method has been validated for eight glucosinolates: glucobrassicin, glucoraphanin, glucoiberin, glucoerucin, progoitrin, gluconapin, sinigrin and glucocheirolin. The linear range was 1–150 µg ml−1, the intra-day and inter-day precision values are within 6% and 8% at the lower limit of quantification, while the overall recovery of the eight glucosinolates was 99 ± 9%. This validated method was used successfully for analysis of glucosinolates content of broccoli sprouts grown in different conditions. Copyright © 2012 John Wiley & Sons, Ltd.