Screening for unknown synthetic steroids in human urine by liquid chromatography-tandem mass spectrometry
Article first published online: 2 JUN 2005
Copyright © 2005 John Wiley & Sons, Ltd.
Journal of Mass Spectrometry
Volume 40, Issue 7, pages 955–962, July 2005
How to Cite
Thevis, M., Geyer, H., Mareck, U. and Schänzer, W. (2005), Screening for unknown synthetic steroids in human urine by liquid chromatography-tandem mass spectrometry. J. Mass Spectrom., 40: 955–962. doi: 10.1002/jms.873
- Issue published online: 20 JUL 2005
- Article first published online: 2 JUN 2005
- Manuscript Accepted: 30 MAR 2005
- Manuscript Received: 3 FEB 2005
- Manfred-Donike Society, Cologne.
- liquid chromatography-mass spectrometry;
- precursor ion scan;
Chemically modified steroids (designer steroids), including tetrahydrogestrinone and norbolethone, pose a threat to the integrity of the sport community. These compounds have recently been detected in urine specimens from athletes, resulting in temporary or permanent suspension from amateur and/or professional competition. Triple quadrupole mass spectrometers enable doping control laboratories to screen for unknown, anabolic, androgenic steroids utilizing precursor ion scans. On the basis of common dissociation patterns of steroids with common structural features, characteristic product ions were selected to serve as diagnostic markers for previously unidentified drugs or drug metabolites in human urine samples. An assay was established to complement standard screening procedures. Urine specimens were enzymically hydrolyzed, partitioned into ether, concentrated, and analyzed by precursor ion scanning. Spectra from samples fortified with eight standard compounds (methyltestosterone, ethyltestosterone, 1-testosterone, gestrinone, dihydrogestrinone, tetrahydrogestrinone, norbolethone, and propyltrenbolone) and one deuterium-labeled analog (d4-tetrahydrogestrinone) at 50 ng/ml of urine, had precursor ion peaks other than those from common endogenous steroids. Subsequent product ion scan experiments on precursor ions of peaks of unknown origin provided structural identification of the unknown compounds. Copyright © 2005 John Wiley & Sons, Ltd.