Special Feature: Perspective
Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology
Article first published online: 19 OCT 2005
Copyright © 2005 John Wiley & Sons, Ltd.
Journal of Mass Spectrometry
Volume 40, Issue 10, pages 1261–1275, October 2005
How to Cite
Takáts, Z., Wiseman, J. M. and Cooks, R. G. (2005), Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology. J. Mass Spectrom., 40: 1261–1275. doi: 10.1002/jms.922
- Issue published online: 19 OCT 2005
- Article first published online: 19 OCT 2005
- Manuscript Accepted: 4 AUG 2005
- Manuscript Received: 21 JUN 2005
- InProteo LLC (Indianapolis, IN).
- Prosolia, Inc. (Indianapolis, IN).
- Thermo Finnigan (San Jose, CA).
- Office of Naval Research.
- electrospray ionization;
- desorption ionization;
- natural products;
- high throughput;
- ionization mechanisms;
- in vivo analysis;
- in situ analysis;
- chemical profiling
Analytical characteristics of DESI are summarized. Examples of applications to small and large molecules, to in situ analysis, and to high-throughput analyses are presented. Evidence is provided for both a heterogeneous charge-transfer mechanism and a droplet pick-up mechanism of ionization. The speed, lack of the need for sample preparation, selectivity, and sensitivity of DESI are all demonstrated and discussed. Instrumentation is also discussed. Forensic applications as well as emerging areas of application including tissue imaging are given emphasis. Copyright © 2005 John Wiley & Sons, Ltd.