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MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression

Authors

  • Gitta Schlosser,

    1. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518
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  • Gabriella Pocsfalvi,

    1. Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, Avellino, 83100, Italy
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  • Emőke Huszár,

    1. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518
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  • Antonio Malorni,

    1. Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, Avellino, 83100, Italy
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  • Ferenc Hudecz

    Corresponding author
    1. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518
    2. Department of Organic Chemistry, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518
    • Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, Budapest 112, Hungary, H-1518.
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  • Paper presented at the 23rd Informal Meeting on Mass Spectrometry, Fiera di Primiero, Italy, 15–19 May 2005.

Abstract

The effect of matrix composition on signal suppression caused by a dominant compound under MALDI ionization was studied using the combinatorial TQTXT pentapeptide library as a model system. The peptide library is composed of 19 components with all proteinogenic amino acids except cysteine in position X. From these compounds, only the Arg peptide (TQTRT) was detected with sufficient intensity in the MALDI-TOF mass spectrum under typical MALDI conditions (CCA matrix). The analysis of a set of compounds utilized as different matrix components, additives and a cationizing agent revealed that the composition of the matrix is a critical point in signal suppression. Highly improved ion yields were achieved by using a CCA/DHB mixture as a matrix. The addition of K+ as a cationizing agent to the CCA matrix resulted in MALDI-TOF mass spectra with relative ion intensities very similar to those obtained by electrospray ionization. Copyright © 2005 John Wiley & Sons, Ltd.

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