PSAQ™ standards for accurate MS–based quantification of proteins: from the concept to biomedical applications (page i)
Guillaume Picard, Dorothée Lebert, Mathilde Louwagie, Annie Adrait, Céline Huillet, François Vandenesch, Christophe Bruley, Jérôme Garin, Michel Jaquinod and Virginie Brun
Article first published online: 7 OCT 2012 | DOI: 10.1002/jms.3111
With the development of the Protein Standard Absolute Quantification (PSAQ method), the use of isotopically labeled analogue standards and MS analysis was transferred from the field of small molecules to the field of proteomics analysis. In this article, Dr Virginie Brun and colleagues focus on the use of PSAQ standards for absolute quantification of biomedically relevant proteins. They start by describing the research project which first led to develop the PSAQ technology and then present the methods used to produce, control the quality of and use these labeled proteins for absolute MS-based quantification. Finally, the main current applications of the PSAQ method are illustrated.