Journal of Mass Spectrometry

Cover image for Vol. 48 Issue 3

March 2013

Volume 48, Issue 3

Pages 291–422

  1. Special feature: perspective

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
    1. Peptide backbone fragmentation initiated by side-chain loss at cysteine residue in matrix-assisted laser desorption/ionization in-source decay mass spectrometry (page i)

      D. Asakawa, N. Smargiasso, L. Quinton and E. De Pauw

      Version of Record online: 12 MAR 2013 | DOI: 10.1002/jms.3186

      Thumbnail image of graphical abstract

      MALDI in-source decay (ISD) was first introduced in 1995 in a landmark paper by RS Brown and JJ Lenon (Anal. Chem. 1995, 67, 3990–3999). Although MALDI MS is considered a soft ionization technique which enables the analysis of high molecular weight proteins exceeding several hundred kDa in an intact manner, it became apparent that some analyte fragmentation occurs on a very short time scale during the desorption ionization process. ISD is the combination of laser induced fragmentation and rapidly occurring metastable decay. This phenomenon can be used to obtain primary sequence information on peptides and (small) proteins also referred as top down sequencing. Beyond its analytical utility, the ISD fragmentation mechanism is currently being studied by several groups. In this perspective special feature article, the MALDI-ISD mechanism is reviewed and a specific fragmentation mechanism is introduced and studied in details: the N-terminal side of Cys residue is described to promote the generation of c' and w ISD fragments. Dr Edwin De Pauw is Professor of Chemistry at Liege University in Belgium. One of the objectives of his mass spectrometry laboratory is to contribute to the development of new fundamental concepts and to enlighten their applications in the fields of analytical chemistry.

  2. Editorial

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
  3. Research articles

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
  4. Application note

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
  5. JMS letters

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
  6. Special feature: perspective

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
  7. Research articles

    1. Top of page
    2. Special feature: perspective
    3. Editorial
    4. Research articles
    5. Application note
    6. JMS letters
    7. Special feature: perspective
    8. Research articles
    1. Sulfides: chemical ionization induced fragmentation studied with Proton Transfer Reaction-Mass Spectrometry and density functional calculations (pages 367–378)

      Erna Schuhfried, Michael Probst, Jumras Limtrakul, Sippakorn Wannakao, Eugenio Aprea, Luca Cappellin, Tilmann D. Märk, Flavia Gasperi and Franco Biasioli

      Version of Record online: 11 MAR 2013 | DOI: 10.1002/jms.3153

    2. Degrees of freedom effect on fragmentation in tandem mass spectrometry of singly charged supramolecular aggregates of sodium sulfonates (pages 379–383)

      Serena Indelicato, David Bongiorno, Sergio Indelicato, Laszlo Drahos, Vincenzo Turco Liveri, Lilla Turiák, Karoly Vékey and Leopoldo Ceraulo

      Version of Record online: 12 MAR 2013 | DOI: 10.1002/jms.3161

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    4. Quantification of L-ergothioneine in human plasma and erythrocytes by liquid chromatography-tandem mass spectrometry (pages 406–412)

      Ling-Zhi Wang, Win-Lwin Thuya, Dorothy Su-Lin Toh, Michael George-Limenta Lie, Jie-Ying Amelia Lau, Li-Ren Kong, Seow-Ching Wan, Kian-Ngiap Chua, Edmund Jon-Deoon Lee and Boon-Cher Goh

      Version of Record online: 12 MAR 2013 | DOI: 10.1002/jms.3150

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