Comparative evaluation of measles virus-specific RT-PCR methods through an international collaborative study
Article first published online: 4 MAR 2003
Copyright © 2003 Wiley-Liss, Inc.
Journal of Medical Virology
Volume 70, Issue 1, pages 171–176, May 2003
How to Cite
Afzal, M.A., Osterhaus, A.D.M.E., Cosby, S.L., Jin, L., Beeler, J., Takeuchi, K. and Kawashima, H. (2003), Comparative evaluation of measles virus-specific RT-PCR methods through an international collaborative study. J. Med. Virol., 70: 171–176. doi: 10.1002/jmv.10371
- Issue published online: 4 MAR 2003
- Article first published online: 4 MAR 2003
- Manuscript Accepted: 7 NOV 2002
- measles virus;
Comparison of RT-PCR assays established in house at various places revealed that laboratories could differ in sensitivity by as much as 1,000-fold in terms of the ability to detect measles virus sequences in clinical samples. The study indicates that PCR findings, positive or negative, are questionable if they are not supported by the associated data demonstrating the overall sensitivity of the assay applied. Measles virus-specific RT-PCR-based assays need to be validated using standard virus preparation or nucleic acid-based target templates. A correlation between real-time quantitative PCR and the conventional PCR for measles virus is highly desirable. J. Med. Virol. 70:171–176, 2003. © 2003 Wiley-Liss, Inc.