Identification of a new genotype H wild-type mumps virus strain and its molecular relatedness to other virulent and attenuated strains

  1. Georgios Amexis1,*,
  2. Steven Rubin1,
  3. Nando Chatterjee2,
  4. Kathryn Carbone1,
  5. Kostantin Chumakov1

Article first published online: 9 APR 2003

DOI: 10.1002/jmv.10392

Issue

Journal of Medical Virology

Journal of Medical Virology

Volume 70, Issue 2, pages 284–286, June 2003

Additional Information

How to Cite

Amexis, G., Rubin, S., Chatterjee, N., Carbone, K. and Chumakov, K. (2003), Identification of a new genotype H wild-type mumps virus strain and its molecular relatedness to other virulent and attenuated strains. Journal of Medical Virology, 70: 284–286. doi: 10.1002/jmv.10392

Author Information

  1. 1

    Center for Biologics Evaluation and Research, FDA, Rockville, Maryland

  2. 2

    Virus Isolation Laboratory, New York State Department of Health, Wadsworth Center, Slingerland, New York

*CBER/FDA, 1401 Rockville Pike HFM-470, Rockville, MD 20852.

Publication History

  1. Issue published online: 9 APR 2003
  2. Article first published online: 9 APR 2003
  3. Manuscript Accepted: 12 NOV 2002

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Keywords:

  • mumps;
  • attenuation;
  • wild-type isolate;
  • genetic stability;
  • quasispecies;
  • vaccine

Abstract

A single clinical isolate of mumps virus designated 88-1961 was obtained from a patient hospitalized with a clinical history of upper respiratory tract infection, parotitis, severe headache, fever and lymphadenopathy. We have sequenced the full-length genome of 88-1961 and compared it against all available full-length sequences of mumps virus. Based upon its nucleotide sequence of the SH gene 88-1961 was identified as a genotype H mumps strain. The overall extent of nucleotide and amino acid differences between each individual gene and protein of 88-1961 and the full-length mumps samples showed that the missense to silent ratios were unevenly distributed. Upon evaluation of the consensus sequence of 88-1961, four positions were found to be clearly heterogeneous at the nucleotide level (NP 315C/T, NP 318C/T, F 271A/C, and HN 855C/T). Sequence analysis revealed that the amino acid sequences for the NP, M, and the L protein were the most conserved, whereas the SH protein exhibited the highest variability among the compared mumps genotypes A, B, and G. No identifying molecular patterns in the non-coding (intergenic) or coding regions of 88-1961 were found when we compared it against relatively virulent (Urabe AM9 B, Glouc1/UK96, 87-1004 and 87-1005) and non-virulent mumps strains (Jeryl Lynn and all Urabe Am9 A substrains). J. Med. Virol. 70: 284–286, 2003. © 2003 Wiley-Liss, Inc.

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