The isolation of enteroviruses from blood: A comparison of four processing methods

Authors

  • Sandra L. Prather,

    1. University of Rochester Medical Center, Departments of Microbiology, Rochester, New York
    Current affiliation:
    1. Clinical Laboratories/Microbiology, Wellborn Baptist Hospital, 401 S.E. Sixth St., Evansville, IN 47713
    Search for more papers by this author
  • Ron Dagan,

    1. Pediatrics, Rochester, New York
    Search for more papers by this author
    • Ron Dagan is the recipient of the University of Rochester-Israeli Pediatric Scholar Exchange

  • Jerri A. Jenista,

    1. Pediatrics, Rochester, New York
    Search for more papers by this author
  • Marilyn A. Menegus PhD

    Corresponding author
    1. Pediatrics, Rochester, New York
    2. Pathology, Rochester, New York
    • Dept of Microbiology, Box 710, University of Rochester Medical Center, Rochester, NY 14642
    Search for more papers by this author

Abstract

Blood from 28 children hospitalized with symptomatic enterovirus infections was processed by four different methods in an effort to define optimum conditions for detecting viremia. Enteroviremia was demonstrated in 11/28 children. Virus was isolated by method 1 (serum) in 7/11 children and by method 2 (mononuclear leukocytes) in 9/11, but in only 3/10 and 3/11 children by methods 3 and 4 (granulocytes and plasma-mixed leukocytes, respectively). In four children, the only blood isolate was from mononuclear leukocytes, and in two, serum was the only positive blood preparation. This suggests that viremia can be often detected in hospitalized children with enterovirus disease and shows that the methods used for processing blood may significantly influence the isolation rate.

Ancillary