Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175
Article first published online: 7 DEC 2005
Copyright © 1987 Wiley Subscription Service, Inc., A Wiley Company
Journal of Medical Virology
Volume 22, Issue 1, pages 35–44, May 1987
How to Cite
Anderson, D. A. (1987), Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175. J. Med. Virol., 22: 35–44. doi: 10.1002/jmv.1890220106
- Issue published online: 7 DEC 2005
- Article first published online: 7 DEC 2005
- Manuscript Accepted: 16 OCT 1986
- National Health and Medical Research Council. D.A.A.
- cytopathic effect;
- heat resistance;
- clonal isolation
Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B-SC-I cells caused a specific cytopathic effect upon acute infection of B-SC-1 cells. The virus formed visible plaques on B-SC-I cell monolayers after 9 to 14 days of incubation at 34°C. and virus can therefore be titrated by plaque assay. Virus could be re-isolated from plaques of infected cells. which allows the clonal isolation of HAV variants. The stability of a plaquepurified variant of HAV at elevated temperatures exceeded that of poliovirus type 1. but this variant is less stable than previously reported strains of HAV.