Conservation of HPV-16 E6/E7 ORF sequences in a cervical carcinoma

Authors

  • Dr. Richard W. Cone,

    Corresponding author
    1. Department of Laboratory Medicine, University of washington, Pacific avenue NE, Seattle, WA and Children's Hospital, Virology Division, 4800 Sand Point way NE, Seattle, Wa 98105
    • Department of Laboratory Medicine, University of washington, Pacific avenue NE, Seattle, WA and Children's Hospital, Virology Division, 4800 Sand Point way NE, Seattle, Wa 98105
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  • Anthony C. Minson,

    1. First Hill Women's Clinic, Seattle, Washington
    Current affiliation:
    1. Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
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  • Michael R. Smith,

    1. First Hill Women's Clinic, Seattle, Washington
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  • James K. McDougall

    1. Fred Hutchinson Cancer Research Center, Seattle, Washington
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Abstract

A cervical carcinoma that contained human papillomavirus (HPV)-l6 homologous DNA was analyzed. Each tumor cell genome contained a single, incomplete copy of HPV-16 DNA. The E6 and E7 open reading frames (ORFs) were com- pletely conserved relative to other published HPV-I6 sequences. Much of the non-coding region (NCR) was free of base changes, including complete conservation of several regulatory elements. Multiple mutations were identified in the remaining integrated HPV-16 DNA, which was composed of parts of the L1 and E1 ORFs. The extraordinary conservation of the E6/E7 DNA sequence, as compared with other regions of the integrated HPV-16 DNA, supports the role of E6/E7 in tumorigenesis. © 1992 Wiley-Liss, Inc.

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