The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL, and GenBank Nucleotide Sequence Databases with the accession numbers D16666 (E88) and D16667 (H2).
Complete nucleotide sequences and the characteristics of two hepatitis B virus mutants causing serologically negative acute or chronic hepatitis B†
Article first published online: 7 DEC 2005
Copyright © 1995 Wiley-Liss, Inc., A Wiley Company
Journal of Medical Virology
Volume 45, Issue 3, pages 247–252, March 1995
How to Cite
Uchida, T., Gotoh, K. and Shikata, T. (1995), Complete nucleotide sequences and the characteristics of two hepatitis B virus mutants causing serologically negative acute or chronic hepatitis B. J. Med. Virol., 45: 247–252. doi: 10.1002/jmv.1890450303
- Issue published online: 7 DEC 2005
- Article first published online: 7 DEC 2005
- Manuscript Accepted: 13 APR 1994
- silent infection;
- hepatitis F;
- X gene;
- X protein
Hepatitis B virus (HBV) DNA was amplified by the polymerase chain reaction from the sera of a patient with acute hepatitis and a patient with chronic hepatitis. Both patients were negative for serum hepatitis B surface antigen and hepatitis B core antibodies and had been previously diagnosed as non-A, non-B, non-C, non-D, non-E hepatitis. The nucleotide sequence revealed an 8-nucleotide deletion in the X-gene coding region creating a C-terminally truncated X protein, and probable mutation of the enhancer 11/core promoter element. In addition, DR2 showed a T-to-C mutation at the extreme 5′-terminus. These mutations within the X-gene coding region must suppress replication and expression of HBV DNA, and this seems to be responsible for absence of serological markers despite the presence of HBV infection. © 1995 Wiley-Uss, inc.