Article

Emergence of hepatitis B virus S gene mutant in a liver transplant recipient

  1. Elisabetta Cariani1,*,
  2. Antonella Ravaggi1,
  3. Elisabetta Tanzi2,
  4. Luisa Romanò2,
  5. Gianfranco Fiordalisi1,
  6. Giorgio Bellati3,
  7. Lucio Caccamo4,
  8. Dinangelo Galmarini4,
  9. Alberto Albertini1,
  10. Alessandro Zanetti2

Article first published online: 7 DEC 2005

DOI: 10.1002/jmv.1890470419

Issue

Journal of Medical Virology

Journal of Medical Virology

Volume 47, Issue 4, pages 410–415, December 1995

Additional Information

How to Cite

Cariani, E., Ravaggi, A., Tanzi, E., Romanò, L., Fiordalisi, G., Bellati, G., Caccamo, L., Galmarini, D., Albertini, A. and Zanetti, A. (1995), Emergence of hepatitis B virus S gene mutant in a liver transplant recipient. J. Med. Virol., 47: 410–415. doi: 10.1002/jmv.1890470419

Author Information

  1. 1

    III Laboratory of Clinical Chemistry and Hospital of Brescia, Institute of Chemistry, School of Medicine, University of Brescia, Brescia, Milan, Italy

  2. 2

    Institute of Virology, University of Milan, Milan, Italy

  3. 3

    Liver Unit, Crespi Division, Ospedale Niguarda, Milan, Italy

  4. 4

    Liver Transplant Unit, Ospedale Maggiore, Milan, Italy

*Institute of Chemistry, C/O III Laboratorio Analisi, Spedali Civili di Brescia, 25123 Brescia, Italy

Publication History

  1. Issue published online: 7 DEC 2005
  2. Article first published online: 7 DEC 2005
  3. Manuscript Accepted: 10 JUN 1995

Funded by

  • Progetto Ricerche Finalizzate. Grant Number: 1346, 1993

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Keywords:

  • HBsAg “a” determinant;
  • monoclonal antibodies;
  • polymerase chain reaction;
  • DNA enzyme immunoassay;
  • escape mutants

Abstract

Immunological and genomic analysis of the “a” determinant was carried out in seven patients with concurrent HBsAg and anti-HBs, four of whom were immunized against hepatitis B virus at liver transplant, two with histologically characterized chronic hepatitis B virus infection, and one HBsAg healthy carrier. The immune reactivity of the HBsAg “a” determinant was evaluated by binding to specific monoclonal antibodies, and the corresponding genomic sequence was studied by differential hybridization in microtiter plates and nucleotide sequence analysis. A double mutation generating an amino acid change (glycine to lysine) at residue 145, able to impair recognition by monoclonal antibodies, was observed in the post-transplant serum from one patient. No significant alteration of the “a” determinant sequence or reactivity was detected in the other patients.

Amino acid residue 145 appears therefore to be critical for the recognition by anti-HBs antibodies. A previously undescribed glycine to lysine substitution at this level interferes with the immune reactivity of the “a” determinant. © Wiley-Liss, Inc.

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