Research Article

A serological survey on neutralizing antibody titer of SARS convalescent sera

  1. Jian-San Zhang1,
  2. Jiang-Ting Chen1,
  3. Yu-Xuan Liu1,
  4. Zhen-Shan Zhang1,
  5. Hong Gao1,
  6. Yan Liu1,
  7. Xu Wang1,
  8. Ye Ning1,
  9. Yu-Fen Liu1,
  10. Qiang Gao1,
  11. Jian-Guo Xu2,
  12. Chuan Qin3,
  13. Xiao-Ping Dong4,
  14. Wei-Dong Yin1,*

Article first published online: 24 AUG 2005

DOI: 10.1002/jmv.20431

Issue

Journal of Medical Virology

Journal of Medical Virology

Volume 77, Issue 2, pages 147–150, October 2005

Additional Information

How to Cite

Zhang, J.-S., Chen, J.-T., Liu, Y.-X., Zhang, Z.-S., Gao, H., Liu, Y., Wang, X., Ning, Y., Liu, Y.-F., Gao, Q., Xu, J.-G., Qin, C., Dong, X.-P. and Yin, W.-D. (2005), A serological survey on neutralizing antibody titer of SARS convalescent sera. Journal of Medical Virology, 77: 147–150. doi: 10.1002/jmv.20431

Author Information

  1. 1

    Sinovac Biotech Co. Ltd., Beijing, China

  2. 2

    Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

  3. 3

    The Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

  4. 4

    Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China

*Sinovac Biotech Co. Ltd., No. 39 Shangdi Xi Rd., Haidian District, Beijing 100085, China.

Publication History

  1. Issue published online: 24 AUG 2005
  2. Article first published online: 24 AUG 2005
  3. Manuscript Accepted: 18 MAY 2005

Funded by

  • Research on Inactivated SARS Vaccine. Grant Number: 2003AA208201

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Keywords:

  • geometric mean titer;
  • immunity;
  • neutralization assay;
  • SARS;
  • SARS-CoV

Abstract

A seroepidemiologic study was conducted in North China in 2003 to determine the neutralizing antibody titer of severe acute respiratory syndrome (SARS) convalescent sera. A total of 99 SARS convalescent serum samples were collected from patients from the Inner Mongolia Autonomous Region, Hebei Province, and Beijing 35–180 days after the onset of symptoms. The anti-SARS antibodies were detected by enzyme-linked immunosorbent assay (ELISA), neutralization assay, and Western blot. Eighty-seven serum samples were confirmed to be positive for SARS antibodies. The neutralizing antibody titer of the 87 positive sera was analyzed quantitatively by neutralization assay. The geometric mean titer (GMT) of the 87 convalescent sera was 1:61. The Kolmogorov–Smirnov test showed that the neutralizing antibody titers conform to normal distribution, which suggests that the average anti-SARS antibody level in this study was representative of the convalescent antibody level of the SARS population. This result could be useful for the development and quality control of SARS vaccines. J. Med. Virol. 77:147–150, 2005. © 2005 Wiley-Liss, Inc.

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