Molecular epidemiology of the hepatitis C virus in Western Siberia

Authors


  • The work was performed in State Research Center of Virology and Biotechnology “Vector,” Koltsovo, Novosibirsk region, Russia.

Abstract

Western Siberia is the region with little information on the prevalence of hepatitis C virus (HCV) infection, genotypic diversity of HCV isolates and risk factors. A molecular epidemiological survey was conducted to clarify these issues. Four groups of volunteers were included in a cross-sectional study (n = 500 in each group): health care workers; daycare patients from a hospital for drug users, daycare patients from an AIDS prevention and control center; and persons admitted to a local general practice clinic for any reason (outpatients). The anti-HCV IgG prevalence was 4.6% in health care workers, 48.0% in a narcological center, 35.8% in AIDS center, and 5.6% in outpatients. HCV RNA was found in 79.3%–86.3% of seropositives. A total of 388 HCV isolates were genotyped by direct sequencing and phylogenetic analysis of the 5′-UTR and NS5B regions of HCV genome. The genotypes distribution was: 1b—50.3%, 2a—4.4%, 2c—0.3%, 3a—44.8%. One isolate (0.3%) could not be typed unambiguously. This genotypic diversity is intermediate between that of European Russia and China. Genotype 1 prevailed in an older age group (75% among 51–60 years old), and genotype 3 was most prevalent in young people (51.4% in 16–20 years old). A statistically significant (P < 0.05) increase in risk was found in intravenous drug users (odds ratio (OR) = 77.5), unemployed persons (OR = 16.3), persons having >4 sexual partners during lifetime (OR = 4.3), and male homosexuals (OR = 6.6). J. Med. Virol. 77:382–389, 2005. © 2005 Wiley-Liss, Inc.

Ancillary