Part of these data has been presented at the recent meeting of The American Association for the Study of Liver Diseases (AASLD 2004, Boston, MA; Hepatology 40 Suppl. 1: abstract 1004). The authors do not have a commercial or other association that might pose a conflict of interest.
Different composition of intrahepatic lymphocytes in the immune-tolerance and immune-clearance phase of chronic hepatitis B†
Version of Record online: 22 MAR 2006
Copyright © 2006 Wiley-Liss, Inc.
Journal of Medical Virology
Volume 78, Issue 5, pages 561–568, May 2006
How to Cite
Sprengers, D., van der Molen, R.G., Kusters, J.G., Hansen, B., Niesters, H.G.M., Schalm, S.W. and Janssen, H.L.A. (2006), Different composition of intrahepatic lymphocytes in the immune-tolerance and immune-clearance phase of chronic hepatitis B. J. Med. Virol., 78: 561–568. doi: 10.1002/jmv.20576
- Issue online: 22 MAR 2006
- Version of Record online: 22 MAR 2006
- Manuscript Accepted: 19 JAN 2006
- The Netherlands Organisation for Scientific Research (NWO). Grant Numbers: 920-03-244, 907-00-021
- fine needle aspiration biopsy;
- viral hepatitis;
- intrahepatic immune response
Based on virological and biochemical parameters patients with chronic hepatitis B virus (HBV) are divided into distinct clinical phases: the immune-tolerance phase, the immune-clearance phase, and the inactive carrier state. Unclear is whether these phases have characteristic intrahepatic immune responses. The composition of liver-derived lymphocytes in patients with chronic HBV infection was studied. In 47 patients the composition of liver-derived lymphocytes was analyzed by flow cytometry of fine needle aspiration biopsies of the liver. The proportion natural killer (NK) cells in the liver was significantly higher in immune-tolerant than in immune-clearance patients and inactive carriers. No differences were found in proportion CD4+ T-cells and CD8+ T-cells, in these phases. However, when patients in the immune-clearance phase, with similar alanine transaminase (ALT), were grouped according to viral load, the proportion CD8+ T-cells was higher in those with high viral load. In contrast, the proportion CD4+ T-cells was increased in patients with low HBV-DNA. These differences were absent in the peripheral blood (PB). Intrahepatic HBV-specific CD8+ T-cells were mainly found in immune-clearance patients with low viral load. In conclusion, clear differences in the intrahepatic cellular infiltrate were found between the various clinical phases of chronic HBV infection. These findings are relevant to the design of new, individualized anti-viral strategies. J. Med. Virol. 78:561–568, 2006. © 2006 Wiley-Liss, Inc.