Reactivation of precore variant hepatitis B virus in a child with severe aplastic anaemia
Article first published online: 8 OCT 2001
Copyright © 2001 Wiley-Liss, Inc.
Journal of Medical Virology
Volume 65, Issue 3, pages 470–472, 1 November 2001
How to Cite
Smith, P.R., Zampino, R., Gutteridge, C., Karayiannis, P. and Aitken, C. (2001), Reactivation of precore variant hepatitis B virus in a child with severe aplastic anaemia. J. Med. Virol., 65: 470–472. doi: 10.1002/jmv.2059
- Issue published online: 8 OCT 2001
- Article first published online: 8 OCT 2001
- Manuscript Accepted: 14 NOV 2000
- precore sequence
Reactivation of hepatitis B e antigen (HBeAg) negative chronic hepatitis B virus (HBV) infection due to selection of precore variant virus is an uncommon complication of previous hepatitis B infection, and virtually unrecognised in children and adolescents. A child who had received treatment with methylprednisolone and antilymphocyte globulin for severe aplastic anaemia developed high levels of detectable HBV DNA associated with hepatitis B e antibody (anti-HBe) positivity. HBV DNA was extracted, amplified and the core and precore regions sequenced from 2 samples. A mixture of wild-type and the precore variants A1896 and A1899 was detected in both samples, with the wild-type predominating in the second sample. Reinfection was excluded by phylogenetic analysis using Phylip and the neighbour-joining method. Precore variant Hepatitis B virus can be transmitted to children as a primary infection, and it is important that aggressive liver disease, particularly in the presence of the anti-HBe phenotype, be investigated. Further studies are needed to determine the frequency of these variants. J. Med. Virol. 65:470–472, 2001. © 2001 Wiley-Liss, Inc.