Polyomavirus BK non-coding control region rearrangements in health and disease
Article first published online: 27 JUN 2007
Copyright © 2007 Wiley-Liss, Inc.
Journal of Medical Virology
Volume 79, Issue 8, pages 1199–1207, August 2007
How to Cite
Sharma, P. M., Gupta, G., Vats, A., Shapiro, R. and Randhawa, P. S. (2007), Polyomavirus BK non-coding control region rearrangements in health and disease. J. Med. Virol., 79: 1199–1207. doi: 10.1002/jmv.20909
- Issue published online: 27 JUN 2007
- Article first published online: 27 JUN 2007
- Manuscript Accepted: 25 APR 2007
- NIH. Grant Numbers: #RO-1 AI 51227, AI 63360, AI 060602
- BK virus;
- non-coding control region
BK virus is an increasingly recognized pathogen in transplanted patients. DNA sequencing of this virus shows considerable genomic variability.
To understand the clinical significance of rearrangements in the non-coding control region (NCCR) of BK virus (BKV), we report a meta-analysis of 507 sequences, including 40 sequences generated in our own laboratory, for associations between rearrangements and disease, tissue tropism, geographic origin, and viral genotype.
NCCR rearrangements were less frequent in (a) asymptomatic BKV viruria compared to patients viral nephropathy (1.7% vs. 22.5%), and (b) viral genotype 1 compared to other genotypes (2.4% vs. 11.2%). Rearrangements were commoner in malignancy (78.6%), and Norwegians (45.7%), and less common in East Indians (0%), and Japanese (4.3%). A surprising number of rearranged sequences were reported from mononuclear cells of healthy subjects, whereas most plasma sequences were archetypal. This difference could not be related to potential recombinase activity in lymphocytes, as consensus recombination signal sequences could not be found in the NCCR region.
NCCR rearrangements are neither required nor a sufficient condition to produce clinical disease. BKV nephropathy and hemorrhagic cystitis are not associated with any unique NCCR configuration or nucleotide sequence. J. Med. Virol. 79: 1199–1207, 2007. © 2007 Wiley-Liss, Inc.