Research Article

Comparison between Sendai virus and adenovirus vectors to transduce HIV-1 genes into human dendritic cells

  1. Noriaki Hosoya1,
  2. Toshiyuki Miura1,
  3. Ai Kawana-Tachikawa1,
  4. Tomohiko Koibuchi1,
  5. Tatsuo Shioda2,
  6. Takashi Odawara3,
  7. Tetsuya Nakamura3,
  8. Yoshihiro Kitamura1,
  9. Munehide Kano4,
  10. Atsushi Kato5,
  11. Mamoru Hasegawa6,
  12. Yoshiyuki Nagai7,
  13. Aikichi Iwamoto1,3,8,9,10,*

Article first published online: 18 JAN 2008

DOI: 10.1002/jmv.21052

Issue

Journal of Medical Virology

Journal of Medical Virology

Volume 80, Issue 3, pages 373–382, March 2008

Additional Information

How to Cite

Hosoya, N., Miura, T., Kawana-Tachikawa, A., Koibuchi, T., Shioda, T., Odawara, T., Nakamura, T., Kitamura, Y., Kano, M., Kato, A., Hasegawa, M., Nagai, Y. and Iwamoto, A. (2008), Comparison between Sendai virus and adenovirus vectors to transduce HIV-1 genes into human dendritic cells. Journal of Medical Virology, 80: 373–382. doi: 10.1002/jmv.21052

Author Information

  1. 1

    Division of Infectious Diseases, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan

  2. 2

    Department of Viral Infections, Research Institute of Microbial Diseases, Osaka University, Osaka, Japan

  3. 3

    Department of Infectious Diseases and Applied Immunology, Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

  4. 4

    AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan

  5. 5

    Department of Virology 3, National Institute of Infectious Diseases, Tokyo, Japan

  6. 6

    DNAVEC Research, Inc., Ibaraki, Japan

  7. 7

    Center of Research Network for Infectious Diseases, Riken, Tokyo, Japan

  8. 8

    International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

  9. 9

    Japan-China Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

  10. 10

    Japan-China Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

*Division of Infectious Diseases, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

Publication History

  1. Issue published online: 18 JAN 2008
  2. Article first published online: 18 JAN 2008
  3. Manuscript Accepted: 5 SEP 2007

Funded by

  • AIDS Research from the Ministry of Health, Labor and Welfare of Japan (partial support)
  • Special Coordination Funds for Promoting Science and Technology of MEXT: Strategic cooperation to control emerging and reemerging infections

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Keywords:

  • viral vector;
  • immuno-genetherapy;
  • AIDS

Abstract

Immuno-genetherapy using dendritic cells (DCs) can be applied to human immunodeficiency virus type 1 (HIV-1) infection. Sendai virus (SeV) has unique features such as cytoplasmic replication and high protein expression as a vector for genetic manipulation. In this study, we compared the efficiency of inducing green fluorescent protein (GFP) and HIV-1 gene expression in human monocyte-derived DCs between SeV and adenovirus (AdV). Human monocyte-derived DCs infected with SeV showed the maximum gene expression 24 hr after infection at a multiplicity of infection (MOI) of 2. Although SeV vector showed higher cytopathic effect on DCs than AdV, SeV vector induced maximum gene expression earlier and at much lower MOI. In terms of cell surface phenotype, both SeV and AdV vectors induced DC maturation. DCs infected with SeV as well as AdV elicited HIV-1 specific T-cell responses detected by interferon γ (IFN-γ) enzyme-linked immunospot (Elispot). Our data suggest that SeV could be one of the reliable vectors for immuno-genetherapy for HIV-1 infected patients. J. Med. Virol. 80:373–382, 2008. © 2008 Wiley-Liss, Inc.

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