1096-9071/asset/cover.gif?v=1&s=0dade998bc6232d09bc91c43270b9d2734a6bca3 "Journal of Medical Virology")
The molecular diagnostic work was performed at the Molecular Diagnostic Laboratory, Living Cell Technologies Ltd, Auckland, New Zealand. PERV serology work was performed at Robert Koch Institute, Berlin, Germany.
1096-9071/asset/JMV_centre.gif?v=1&s=0cfbd8a2692b71d14e1d2a1936e4903513e31ac5)
Research Article
Absence of transmission of potentially xenotic viruses in a prospective pig to primate islet xenotransplantation study†
Article first published online: 23 SEP 2008
DOI: 10.1002/jmv.21272
Copyright © 2008 Wiley-Liss, Inc.
Additional Information
How to Cite
Garkavenko, O., Dieckhoff, B., Wynyard, S., Denner, J., Elliott, R. B., Tan, P. L. and Croxson, M. C. (2008), Absence of transmission of potentially xenotic viruses in a prospective pig to primate islet xenotransplantation study. Journal of Medical Virology, 80: 2046–2052. doi: 10.1002/jmv.21272
- †
Publication History
- Issue published online: 23 SEP 2008
- Article first published online: 23 SEP 2008
- Manuscript Accepted: 27 MAY 2008
- Abstract
- References
- Cited By
Keywords:
- xenozoonozes;
- porcine viruses;
- primate study;
- porcine endogenous retrovirus;
- porcine cytomegalovirus;
- porcine lymphotropic herpesvirus
Abstract
Shortage of human donor organs for transplantation has prompted usage of animals as an alternative donor source. Pigs are the most acceptable candidate animals but issues of xenozoonoses remain. Despite careful monitoring of designated pathogen free pigs there is still a risk that their tissues may carry infectious agents. Thus xenotransplantation requires extensive pre-clinical study on safety of the graft especially for those viruses that are either potentially oncogenic and/or immunosuppressive, or can establish persistent infection. A prospective pig-to-primate islet xenotransplantation study was performed which includes monitoring for potentially xenotic viruses namely porcine endogenous retrovirus (PERV), porcine cytomegalovirus (PCMV), porcine lymphotropic herpesvirus (PLHV), and porcine circovirus (PCV) using both molecular diagnostic—PCR and RT-PCR and serology methods. There was no evidence of pig virus transmission into primate recipients. This preclinical study underlines the information concerning viral safety of islet cell xenograft in pig-to-primate xenotransplantation. J. Med. Virol. 80:2046–2052, 2008. © 2008 Wiley-Liss, Inc.