Full genome characterization of hepatitis B virus strains from blood donors in Iran
Article first published online: 18 APR 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Medical Virology
Volume 83, Issue 6, pages 948–952, June 2011
How to Cite
Garmiri, P., Rezvan, H., Abolghasemi, H. and Allain, J.-P. (2011), Full genome characterization of hepatitis B virus strains from blood donors in Iran. J. Med. Virol., 83: 948–952. doi: 10.1002/jmv.21772
- Issue published online: 18 APR 2011
- Article first published online: 18 APR 2011
- Manuscript Accepted: 25 JAN 2010
- National Health Service Blood and Transplant, UK (partial support). Grant Number: BS05/02
- molecular characterization;
Iran is a low to medium endemic country for hepatitis B virus (HBV), depending on the region, where genotype D is dominant. Samples from 170 asymptomatic HBsAg-positive blood donors were quantified and the median viral load was 6.7 × 102 IU/ml with 10.6% samples unquantifiable. Fifty complete genome sequences of these strains were characterized. Phylogenetic analysis identified 98% strains as subgenotype D1 and 2% as D2. Deduced serotypes were ayw2 (94%), ayw1 (4%), and adw (2%). The nucleotide diversity of the complete genome subgenotype D1 Iranian strains was limited (2.8%) and comparison with D1 strains from Egypt and Tunisia revealed little variation between strains from these three countries (range 1.9–2.8%). The molecular analysis of the individual genes revealed that the G1896A mutation was present in 86.2% of the strains and in 26 strains (29.9%) this mutation was accompanied by the G1899A mutation. The double mutations A1762T/G1764A and G1764T/C1766G were found in 20.7% and 24.1% of the strains, respectively. The pre-C initiation codon was mutated in five strains (5.8%). One strain had a 2-amino acid (aa) insertion at position s111 and another sP120Q substitution suggesting a vaccine escape mutant. J. Med. Virol. 83:948–952, 2011. © 2011 Wiley-Liss, Inc.