Molecular and clinical characteristics of hepatitis B virus in Korea
Article first published online: 25 MAY 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Medical Virology
Volume 82, Issue 7, pages 1126–1134, July 2010
How to Cite
Ahn, S. H., Yuen, L., Han, K.-H., Littlejohn, M., Chang, H. Y., Damerow, H., Ayres, A., Heo, J., Locarnini, S. and Revill, P. A. (2010), Molecular and clinical characteristics of hepatitis B virus in Korea. J. Med. Virol., 82: 1126–1134. doi: 10.1002/jmv.21844
- Issue published online: 25 MAY 2010
- Article first published online: 25 MAY 2010
- Manuscript Accepted: 25 JAN 2010
- GlaxoSmithKline Research Fund of the Korean Association for the Study of Liver
- Good Health R&D Project from the Ministry for Health, Welfare and Family Affairs, Republic of Korea. Grant Number: A050021
- hepatitis B virus;
- complete genome;
- disease progression
Korea is an endemic area of hepatitis B virus (HBV) infection but very little is known about the molecular characteristics of HBV isolates from Korean patients or the association with disease progression. The complete HBV genome sequences from 53 Korean patients with chronic hepatitis B, advanced cirrhosis, or hepatocellular carcinoma (HCC) were analyzed to identify (i) subgenotype distribution and genetic diversity and (ii) signature mutations associated with liver disease progression. With the exception of 1 patient infected with HBV/B, all 52 patients (98.1%) were infected with HBV/C, subgenotype C2. These strains were 98.4% identical and the frequency of amino acid substitutions occurring within key immunological epitopes increased with disease severity. A number of amino acid/nucleotide substitutions were associated with HCC, namely sR24K (HBsAg), SI126T (HBsAg), and pcA1846T (precore gene) mutations (P = 0.029, 0.001, and 0.008, respectively). HBV harboring deletions in the pre-S region were also associated with increased liver disease severity (chronic hepatitis B vs. cirrhosis, P = 0.040; chronic hepatitis B vs. HCC, P = 0.040). Despite the high degree of sequence conservation, several key HBV mutations were associated with disease progression. Prospective studies with larger cohorts of patients are required to evaluate further the clinical manifestation of HBV/C2 in Korea. J. Med. Virol. 82: 1126–1134, 2010. © 2010 Wiley-Liss, Inc.