The work was performed at the Wadsworth Center, New York State Department of Health.
Article first published online: 19 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Medical Virology
Volume 83, Issue 12, pages 2172–2181, December 2011
How to Cite
Dupuis, M., Hull, R., Wang, H., Nattanmai, S., Glasheen, B., Fusco, H., Dzigua, L., Markey, K. and Tavakoli, N. P. (2011), Molecular detection of viral causes of encephalitis and meningitis in New York State. J. Med. Virol., 83: 2172–2181. doi: 10.1002/jmv.22169
Conflict of interest: None.
- Issue published online: 19 OCT 2011
- Article first published online: 19 OCT 2011
- Manuscript Accepted: 1 JUN 2011
- New York State International Training and Research Program. Grant Numbers: 1D43TW007384-01, 2D43TW000233-11
- NIH Fogarty International Center
- Centers for Disease Control and Prevention (CDC). Grant Number: U01/CI000311
- central nervous system;
- cerebrospinal fluid;
- polymerase chain reaction
The etiology of encephalitis and meningitis, serious diseases of the central nervous system (CNS), in most cases remains unknown. The importance of establishing a diagnosis however, becomes even more important as advances are made in effective therapy. Molecular methods of detection, in particular, PCR, are being used routinely and have established a place in the arsenal of tools for diagnosis of CNS infections. In this study a viral etiological agent was detected by PCR in 340 of the total 2,357 specimens from patients who exhibited symptoms of encephalitis or meningitis. The detection rate increased from 8.9% during the first year of the study to 14.8% during the second year of the study with improved methodology and an expanded panel of viral agents. Methods were enhanced by developing real-time PCR assays (some multiplexed), using increased automation, superior nucleic acid extraction, and reverse transcription (RT) methods, and incorporation of an internal extraction control. Additionally, adenovirus and human herpes virus 6 (HHV-6) were added to the original panel of 10 viruses that included enteroviruses, herpesviruses, and arboviruses. The most common viruses detected were enteroviruses (129; 5.5%), Epstein-Barr virus (EBV) (85; 3.6%), herpes simplex viruses (HSVs) 1 and 2 (67; 2.8%), and varicella zoster virus (VZV) (44; 1.9%). J. Med. Virol. 83:2172–2181, 2011. © 2011 Wiley Periodicals, Inc.