Genotyping of human rotaviruses circulating among children with diarrhea in Valencia, Venezuela

Authors

  • Esmeralda Vizzi,

    Corresponding author
    1. Virus Biology Laboratory, Center for Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Edo. Miranda, Venezuela
    • Laboratorio de Biología de Virus, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Carretera Panamericana Km. 11, Caracas, 1020-A. Edo. Miranda, Venezuela.
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  • Oscar Piñeros,

    1. Virus Biology Laboratory, Center for Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Edo. Miranda, Venezuela
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  • Germán Gabriel González,

    1. Departamento de Microbiología, Escuela de Ciencias Biomédicas y Tecnológicas, Facultad de Ciencias de la Salud, Universidad de Carabobo (UC), Valencia, Edo. Carabobo, Venezuela
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  • José L. Zambrano,

    1. Virus Biology Laboratory, Center for Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Edo. Miranda, Venezuela
    Current affiliation:
    1. Veterinary Molecular Biology, Montana State University, Bozeman, Montana.
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  • Juan E. Ludert,

    1. Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, Mexico DF, Mexico
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  • Ferdinando Liprandi

    1. Virus Biology Laboratory, Center for Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Edo. Miranda, Venezuela
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  • Conflict of interest statement: The authors declare no conflict of interest.

Abstract

Rotavirus infection is the most common cause of severe gastroenteritis during childhood worldwide, especially in developing countries. Two rotavirus vaccines are available for childhood immunization programs. Evaluation of the vaccine performance will benefit from knowledge of the epidemiological features of rotavirus infection in regional settings. Limited information on the molecular characteristics of the rotavirus types circulating in Venezuela is available. Eighty seven (89.7%) of the 97 ELISA rotavirus positive stool samples collected from children with diarrhea aged <5 years during 2003 in Valencia (Carabobo State), were G-, P- and NSP4-genotyped by RT-PCR and/or automated sequencing. Four common combinations, G3P[8]/NSP4-E1, G2P[4]/NSP4-E2, G9P[8]/NSP4-E1, and G1P[8]/NSP4-E1 were responsible for 50.6%, 35.6%, 5.7%, and 1.1%, respectively of cases of rotavirus diarrhea, most of them (66%) in children ≤12 months. One uncommon G8P[14]/NSP4-E2 strain was also detected. Temporal fluctuation of genotype distribution occurred, but no differences by age, diarrhea severity score, sex, treatment type or patient medical attention were observed, except for the G3P[8]/NSP4-E1, associated with a more severe dehydration than any other type (P < 0.01). The results confirm the broad diversity among rotavirus strains circulating in Venezuela prior to vaccine implementation, showing the predominance of G3, significant proportion of G2 and moderate circulation of G9 strains. Epidemiological surveillance is needed to detect the emergence of new genotypes that could escape protection induced by vaccination. J. Med. Virol. 83:2225–2232, 2011. © 2011 Wiley Periodicals, Inc.

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