Conflicts of interest: None.
Article first published online: 12 APR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Medical Virology
Volume 84, Issue 6, pages 986–991, June 2012
How to Cite
Kawamura, Y., Sugata, K., Nakai, H., Asano, Y., Ohashi, M., Kato, T., Nishimura, N., Ozaki, T., Yui, A., Taniguchi, K. and Yoshikawa, T. (2012), Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus. J. Med. Virol., 84: 986–991. doi: 10.1002/jmv.23296
This study was approved by the review boards of all four institutions (#08-177).
- Issue published online: 12 APR 2012
- Article first published online: 12 APR 2012
- Manuscript Accepted: 7 MAR 2012
- Ministry of Education, Culture, Sports, Science (Strategic Research Base Development Program for Private Universities) and Technology, the Ministry of Health, Labour and Welfare. Grant Numbers: H21-Shinko-009, H20-Kokoro-021
Rotavirus (RV) antigenemia has been reported in patients with gastroenteritis; however, the exact mechanism remains unclear. In order to elucidate the mechanism of RV antigenemia, an association between RV antigenemia and matrix metalloproteinase (MMP) were analyzed. The object of this study was to elucidate the role of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in the pathogenesis of RV antigenemia. Forty children admitted to hospital with RV gastroenteritis were enrolled in this study. Paired serum samples were collected at the time of admission and discharge. Enzyme-linked immunosorbent assays (ELISA) were used to detect serum concentrations of viral antigens, MMP-1, -2, -9, -13, TIMP -1, and -2. Cytokines were measured using flow cytometric beads array. RV antigens were significantly higher in serum collected at the time of admission than discharge (P < 0.001). MMP-9 concentrations were significantly higher in serum collected at the time of admission than discharge (P < 0.001). MMP-2 concentrations were significantly lower in serum collected at the time of admission than discharge (P < 0.001). A weak but a significantly positive association (P = 0.034) was observed between RV antigen and MMP-9 in serum collected at the time of admission, and inverse association was observed between RV antigen and MMP-2. In addition, a weak but significantly positive association (P = 0.002) was observed between IL-6 and MMP-9. These data suggest that MMPs may contribute to the pathogenesis of RV antigenemia. J. Med. Virol. 84:986–991, 2012. © 2012 Wiley Periodicals, Inc.