This is a US government work, and, as such, is in the public domain of The United States of America.
Characterization of ELISA detection of broad-spectrum anti-Epstein–Barr virus antibodies associated with nasopharyngeal carcinoma†
Article first published online: 21 DEC 2012
Puiblished 2012. This is a US government work, and, as such, is in the public domain of The United States of America.
Journal of Medical Virology
Volume 85, Issue 3, pages 524–529, March 2013
How to Cite
Chang, C., Middeldorp, J., Yu, K. J., Juwana, H., Hsu, W.-L., Lou, P.-J., Wang, C.-P., Chen, J.-Y., Liu, M.-Y., Pfeiffer, R. M., Chen, C.-J. and Hildesheim, A. (2013), Characterization of ELISA detection of broad-spectrum anti-Epstein–Barr virus antibodies associated with nasopharyngeal carcinoma. J. Med. Virol., 85: 524–529. doi: 10.1002/jmv.23498
- Issue published online: 22 JAN 2013
- Article first published online: 21 DEC 2012
- Manuscript Accepted: 19 NOV 2012
- Intramural Research Program of the National Cancer Institute
- National Institutes of Health
- Epstein–Barr virus;
- nasopharyngeal carcinoma;
Epstein–Barr virus (EBV) infection is associated with undifferentiated nasopharyngeal carcinomas (NPC). A distinct seroreactivity pattern to EBV is predictive of subsequent risk of sporadic and familial nasopharyngeal carcinomas. There are currently no accepted screening tools for guiding the clinical management of individuals at high-risk for nasopharyngeal carcinomas, particularly unaffected relatives from nasopharyngeal carcinoma multiplex families. Therefore, the reproducibility of a panel of largely synthetic peptide-based anti-EBV antibody ELISAs was evaluated and their ability to distinguish nasopharyngeal carcinoma cases from controls was explored. IgG and IgA antibodies against 6 different EBV antigens (10 assays, total) were tested on sera from 97 individuals representing the full spectrum of anti-EBV seroprevalence (i.e., healthy individuals with no known EBV seroreactivity, healthy individuals with known EBV seroreactivity, and nasopharyngeal carcinoma cases). Each specimen was tested in triplicate to assess within-batch and across-batch variation, and the triplicate testing was repeated on two separate days. Reproducibility was assessed by the coefficients of variation (CVs) and intraclass correlation coefficients (ICCs). All markers were detectable in 17% or more of samples. For all but one marker, the overall, within-batch, and across-batch CVs were below 15%, and the ICCs were above 70% for all but three markers. Sensitivity of these markers to detect prevalent nasopharyngeal carcinomas ranged from 22% to 100%, and among unaffected controls, most distinguished those with and without known seropositivity. In conclusion, a large number of EBV markers can be measured reliably in serum samples using peptide-based anti-EBV ELISAs. J. Med. Virol. 85:524–529, 2013. Puiblished 2012. This is a US government work, and, as such, is in the public domain of The United States of America.