Marike van Hengel and Saskia Wolt are currently on leave from the Medical Faculty, the Vrije University of Amsterdam, Netherlands.
Effect of glutamine and GABA on [U-13C]glutamate metabolism in cerebellar astrocytes and granule neurons
Version of Record online: 30 NOV 2001
Copyright © 2001 Wiley-Liss, Inc.
Journal of Neuroscience Research
Volume 66, Issue 5, pages 885–890, 1 December 2001
How to Cite
Qu, H., Konradsen, J. R., van Hengel, M., Wolt, S. and Sonnewald, U. (2001), Effect of glutamine and GABA on [U-13C]glutamate metabolism in cerebellar astrocytes and granule neurons. J. Neurosci. Res., 66: 885–890. doi: 10.1002/jnr.10055
- Issue online: 30 NOV 2001
- Version of Record online: 30 NOV 2001
- Manuscript Accepted: 21 AUG 2001
- Manuscript Revised: 20 AUG 2001
- Manuscript Received: 17 AUG 2001
- Research Council of Norway. Grant Number: SIP 782008.01
- SINTEF Unimed Foundation
- cerebellar astrocytes;
- cerebellar granule neurons;
- MR spectroscopy
To probe the effect of glutamine and GABA on metabolism of [U-13C]glutamate, cerebellar astrocytes were incubated with [U-13C]glutamate (0.5 mM) in the presence and absence of glutamine (2.5 mM) or GABA (0.2 mM). It could be shown that consumption of [U-13C]glutamate was decreased in the presence of glutamine and release of labeled aspartate and [1,2,3-13C]glutamate decreased as well, whereas the concentrations of these metabolites increased inside the cells. Glutamine decreased energy production from [U-13C]glutamate presumably by substituting for glutamate as an energy substrate. No additional effect was seen in the presence of both glutamine and GABA. When cerebellar granule neurons were incubated with [U-13C]glutamate (0.25 mM) and GABA (0.05 mM), less [U-13C]glutamate was used for energy production than in controls. Because the barbiturate thiopental did not elicit such response (Qu et al., 2000, Neurochem Int 37:207–215) it appears that GABA also has a metabolic function in the glutamatergic cerebellar granule neurons in contrast to the astrocytes. © 2001 Wiley-Liss, Inc.