Get access

Expression of the green fluorescent protein in the oligodendrocyte lineage: A transgenic mouse for developmental and physiological studies

Authors

  • Xiaoqing Yuan,

    1. Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
    Search for more papers by this author
  • Ramesh Chittajallu,

    1. Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
    Search for more papers by this author
  • Shibeshih Belachew,

    1. Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
    Search for more papers by this author
  • Stacie Anderson,

    1. Gene Transfer Laboratory, Hematopoiesis Section, Flow Cytometry Core Unit, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland
    Search for more papers by this author
  • Chris J. McBain,

    1. Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
    Search for more papers by this author
  • Vittorio Gallo

    Corresponding author
    1. Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
    2. Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, Washington, DC
    • Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, 111 Michigan Avenue, N.W., Washington, DC 20010-2970
    Search for more papers by this author

Abstract

We generated a transgenic mouse expressing the enhanced green fluorescent protein (EGFP) under the control of the 2′-3′-cyclic nucleotide 3′-phosphodiesterase (CNP) promoter. EGFP+ cells were visualized in live tissue throughout embryonic and postnatal development. Immunohistochemical analysis in brain tissue and in sciatic nerve demonstrated that EGFP expression was restricted to cells of the oligodendrocyte and Schwann cell lineages. EGFP was also strongly expressed in “adult” oligodendrocyte progenitors (OPs) and in gray matter oligodendrocytes. Fluorescence-activated cell sorting allowed high-yield purification of EGFP+ oligodendrocyte-lineage cells from transgenic brains. Electrophysiological patch clamp recordings of EGFP+ cells in situ demonstrated that OP cells displayed large outward tetraethylammonium (TEA)-sensitive K+ currents and very small inward currents, whereas mature oligodendrocytes were characterized by expression of large inward currents and small outward K+ currents. The proliferation rate of EGFP+ cells in developing white matter decreased with the age of the animals and was strongly inhibited by TEA. Oligodendrocyte development and physiology can be studied in live tissue of CNP-EGFP transgenic mice, which represent a source of pure EGFP+ oligodendrocyte-lineage cells throughout development. © 2002 Wiley-Liss, Inc.

Ancillary